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GDoQ: A Web Server for Predicting Inhibitors Against Bacterial Target GlmU

Welcome to the official documentation for GlmUpred, a specialized computational resource developed to identify and design inhibitors against the bacterial GlmU protein. The GlmU protein is a bifunctional enzyme essential for the synthesis of peptidoglycan and lipopolysaccharide in bacteria, making it an attractive target for developing new antibacterial agents, particularly against drug-resistant strains like Mycobacterium tuberculosis.

Web Server: https://webs.iiitd.edu.in/raghava/gdoq


Citation

Singla, D., Anurag, M., Dash, D., & Raghava, G. P. S. (2011). A web server for predicting inhibitors against bacterial target GlmU protein. BMC Pharmacology, 11, 5. https://doi.org/10.1186/1471-2210-11-5

Zenodo:-(https://doi.org/10.5281/zenodo.20140080)


About the Platform

The emergence of drug-resistant bacteria poses a global health threat. GlmUpred addresses this by providing a platform to predict the inhibitory activity ($IC_{50}$) of chemical compounds against the GlmU protein. The platform utilizes both Quantitative Structure-Activity Relationship (QSAR) and molecular docking techniques to provide a multi-faceted evaluation of potential inhibitors.

Key Features

  • QSAR Modeling: Predicts inhibitory activity based on molecular descriptors calculated from the chemical structure.
  • Docking Integration: Incorporates docking energies into the prediction models to account for the structural fit within the GlmU active site.
  • Diverse Training Data: Models were trained on 84 diverse GlmU inhibitors retrieved from PubChem BioAssay.

Technical Overview

The performance of GlmUpred is based on sophisticated machine learning models that link chemical features to biological activity.

Model Type Descriptors Correlation ($r$)
Docking-based AutoDock Energies 0.35
Descriptor-based CDK & PaDEL Descriptors 0.81
Hybrid Mixed Descriptors 0.81

Model Functionality

  • Activity Prediction: Users can submit the structure of a chemical compound (in SMILES or SDF format) to predict its potential $pIC_{50}$ against GlmU.
  • Molecular Descriptors: The server calculates a wide range of descriptors including constitutional, topological, and geometric properties.
  • Target Specificity: Specifically optimized for the C-terminal uridylyltransferase domain of the GlmU protein.

Applications

  • Antibiotic Discovery: Screening large chemical libraries to find novel leads against tuberculosis and other bacterial infections.
  • Lead Optimization: Assisting medicinal chemists in modifying compounds to increase their potency against the GlmU target.
  • Computational Biology: Providing a specialized tool for studying the structure-activity relationships of bacterial enzyme inhibitors.

Contact & Authors

Prof. Gajendra P. S. Raghava Bioinformatics Centre, Institute of Microbial Technology, Sector 39A, Chandigarh, India. Email: raghava@imtech.res.in


License

This resource is open-access and distributed under the terms of the Creative Commons Attribution License, permitting unrestricted use and distribution provided the original work is properly credited.

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A web server for predicting inhibitors against bacterial target GlmU protein

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