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PopPUNK (Population Partitioning Using Nucleotide Kmers) is a tool for rapid bacterial genome clustering that uses variable-length k-mer distances to define and assign strains at population scale. The --create-db subcommand is the first step in the PopPUNK workflow, taking a set of input assemblies and generating a database of k-mer sketches along with a matrix of pairwise core and accessory distances. This database is then used by downstream PopPUNK commands (such as --fit-model or poppunk_assign) to cluster genomes into strains or assign new queries to existing clusters.
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