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🧪 Aloe Vera Bioactive Extraction Optimizer: A Circular Bioeconomy Approach


🚀 Interactive Optimization Tool

Launch the dashboard to interactively explore the response surfaces and identify the optimal extraction parameters for each solvent system.

Open in Streamlit

📍 Note: For the best experience, use a desktop browser to interact with the 3D models.


💎 Project Overview & Intellectual Property

This project showcases the digital transformation of a patented extraction process designed to valorize Aloe vera processing by-products. By applying Circular Economy principles, we transform discarded Aloe rind into a high-value cosmetic ingredient.

Key Innovations:

  • Patented Technology: Co-inventor of the industrial method for standardized aloesin extraction. 🔗 Open Patent
  • Green Chemistry: Optimized use of "Generally Recognized as Safe" (GRAS) alternative solvents: Ethanol, Propylene Glycol, and Glycerol.
  • Target Bioactivity: Extracts optimized for skin-depigmenting formulations due to their potent anti-tyrosinase activity.

🌿 Scientific Background

The industrial processing of Aloe vera (L.) Burm. f. generates vast amounts of rind as a byproduct. This project uses Response Surface Methodology (RSM) to bridge the gap between laboratory research and industrial application.

Reference: Añibarro-Ortega, M., et al. (2021). Biology, 10(10), 951.

📊 Technical Methodology

The optimization was performed using a Central Composite Rotatable Design (CCRD) to maximize the recovery of Aloesin, which was precisely quantified using HPLC-DAD-ESI/MSn.

Target Response: Aloesin Content: Expressed in mg/g of extract.

Process Variables:

  1. Time ($t$): 10–210 min
  2. Temperature ($T$): 25–95 °C
  3. Solvent Concentration ($S$): 0–100 % (w/w)

Validated Optimization Models

Solvent System Time (min) Temp (°C) Solvent (%) Max Yield(pred) (mg/g) Max Yield(exp) (mg/g) R2adj
Ethanol/Water 92.9 55.9 0.0 48 ± 1 51 ± 4 0.9862
Propylene Glycol/Water 12.0 60.6 51.1 63 ± 2 65 ± 4 0.8976
Glycerol/Water 42.2 56.8 17.5 57 ± 2 61 ± 3 0.8699

🚀 Business & Industrial Impact

  • Waste Valorization: Converts a low-cost byproduct into a high-margin cosmetic active.
  • Cost Reduction: Identifies precise T/t thresholds to optimize ROI and energy consumption.
  • Proven Innovation: Based on a patented process, ensuring a unique competitive advantage.

🛠️ Tech Stack

  • Python 3.x
  • Streamlit (Interactive Dashboard)
  • Statsmodels (OLS Regression & Cubic Modeling)
  • Plotly (3D Surface Visualization)

About

Predictive modeling and process optimization of Aloe vera extraction using experimental design and multivariate polynomial regression.

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