DBCW v1.0.0 - Thesis Release

This release corresponds to the version of the Database Creation Workflow (DBCW) used in:

Freiheit, M.N. (2025). Development of Reproducible Metagenomic Approaches for Skin and Wound Microbiome Analysis. University of Hull.

DBCW is a Snakemake-based workflow developed to build the reference databases required for metagenomic taxonomic and alignment-based analyses. It functions as a modular subworkflow within MMCAW but can also be executed independently.

Key features:

• Automated construction of Kraken2, CAT, and BLAST databases
• Retrieval and preparation of reference sequences and taxonomy data
• Standardised output structure for downstream workflow compatibility
• Version tracking and checksum logging for reproducibility
• Fully reproducible execution via Snakemake and Conda

This release is archived to ensure reproducibility of all analyses presented in the thesis. Users should refer to the README and configuration documentation for usage details.