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Experimental Design and Goals

This repository contains the code to re-analyze single cell RNAseq data previously published here: Yao, H. et al. Timing and cell specificity of senescence drives postnatal lung development and injury. Nat. Commun. 14, 273 (2023) and here: Scaffa, A. et al. Single-cell transcriptomics reveals lasting changes in the lung cellular landscape into adulthood after neonatal hyperoxic exposure. Redox Biol. 48, 102091 (2021)

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  • metadata: Contains Dockerfile to recreate environment for running the notebook.
  • notebooks: Contains Analysis.Rmd notebook to reanalyze data and regenerate figures and tables.
  • data: Contains intermediate figures and tables for publication.

How to reproduce

The Dockerfile can be re-built or pulled from Dockerhub at jwalla12/dennery_scrna_reanalysis:20260319-4. The all_data.combined R object is on Zenodo at https://zenodo.org/records/19118171

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