Timberlake_GHG_mesocosm

This repository contains open-source code, data, & text files.

For information regarding the project, please visit:

Bledsoe, R.B., Finlay, C.G., and Peralta, A.L. (2026). Wetland soil history shapes microbial community composition, while hydrologic disturbance alters greenhouse gas fluxes. Accepted at Ecosphere. bioRxiv. PDF

Finlay, C.G. and Peralta, A.L., 2025. Hydrologic history and flooding pulses control iron and sulfur metabolic composition with variable associations to greenhouse gas production in a coastal wetland mesocosm experiment. bioRxiv. PDF

Peralta, A.L., Bledsoe, R.B., Muscarella, M.E., Huntemann, M., Clum, A., Foster, B., Foster, B., Roux, S., Palaniappan, K., Varghese, N. and Mukherjee, S., 2020. Metagenomes from Experimental Hydrologic Manipulation of Restored Coastal Plain Wetland Soils (Tyrell County, North Carolina). Microbiology resource announcements, 9(41), pp.e00882-20. PDF

Raw amplicon sequence files can be found at NCBI SRA BioProject ID PRJNA636184.

Metagenome sequence files can be found at NCBI SRA BioProject ID PRJNA641216.

We address the following questions

• Aim: To what extent do hydrologic status and plant presence influence microbial biodiversity and ecosystem functions related to biogenic greenhouse gas emissions?

Repo Contents

• analyses: R Markdown files that include R script written by Regina Bledsoe, Colin Finlay, Ariane Peralta, and Mario Muscarella containing functions used in analysis of GHG, soil, and microbial sequence data:

  1. metaG_in_depth.Rmd: metagenomic analysis of Fe, S, C, and N cycling gene modules, for manuscript in review
  2. SoilPhys_GHGs_16S.Rmd: 16S, edaphic, and greenhouse gas analysis, for manuscript in review
  3. GHG_LME.Rmd: linear-mixed effects models of greenhouse gases, for manuscript in review
  4. Bins_Heatmaps.Rmd: Heatmaps of functional genes in metagenome bins, for manuscript in review
  5. /Previous_Versions: collection of previous .Rmd versions, not a part of manuscript in review

• bin:

  • MothurTools.R: An R script written by Mario Muscarella (Indiana University, now at University of Alaska Fairbanks) containing functions used in the analysis of community sequence data.

• data: Files associated with GHG, soil, and microbial data sets.

• figures: Figures (of GHG, soil, microbes-16S rRNA amplicon, microbes-shotgun metagenomes) generated according to R script located in R Markdown file.

• supplemental: Additional sequencing methods information and tables of metagenomic sequence data

Funding Sources

This work was supported by East Carolina University and an NSF GRFP to R. Bledsoe, grant no. DGE-2125684 to C.G.F., and grants DEB #1845845 and CNH2 #2009185 awarded to A.L.P. The metagenomes were produced by the DOE JGI under the Community Science Program (CSP) (JGI CSP grant 503952). The work conducted by the DOE JGI, a DOE Office of Science User Facility, is supported under contract DE-AC02-05CH11231.

Contributors

Dr. Regina Bledsoe (website): Principal Investigator, Former PhD Student in the Peralta Lab at East Carolina University

Colin Finlay: PhD Candidate in the Peralta Lab at East Carolina University

Dr. Mario Muscarella (website): Assistant Professor, University of Alaska Fairbanks

Dr. Ariane Peralta (website): Associate Professor, Department of Biology, East Carolina University