This repository contains open-source code, data, & text files.
For information regarding the project, please visit: Bledsoe RB, Bean EZ, Austin S, and Peralta AL. 2020. A microbial perspective on balancing trade-offs in ecosystem functions in a constructed stormwater wetland. Ecological Engineering. 158. PDF
Raw sequence files can be found at NCBI SRA BioProject ID PRJNA613095.
- Aim: How can wetland design be optimized to enhance ecosystem services and reduce ecosystem disservices?
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analyses: An R Markdown file that includes R script written by Regina Bledsoe, Ariane Peralta, and Mario Muscarella containing functions used in analysis of GHG, soil, and microbial sequence data.
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bin:
- MothurTools.R: An R script written by Mario Muscarella (Indiana University, now at University of Illinois) containing functions used in the analysis of community sequence data.
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data: Files associated with GHG, soil, and microbial data sets.
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figures: Figures (of GHG, soil, microbes) generated according to R script located in R Markdown file.
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mothur: Files containing script for bioinformatic analysis of 16S rRNA gene sequences (Illumina MiSeq platform) using mothur pipeline.
This work was supported by the East Carolina University (to ALP), the National Science Foundation (to RBB), and the North Carolina Sea Grant and Water Resources Research Institute Fellowship to RBB.
Dr. Regina Bledsoe (website): Principal Investigator, PhD Student in the Peralta Lab at East Carolina University
Dr. Eban Bean (website): Co-Principal Investigator, Assistant Professor, Department of Agricultural and Biological Engineering, University of Florida
Samuel Austin: Undergraduate Research Assistant, Student in the Peralta Lab at East Carolina University
Dr. Ariane Peralta (website): Co-Principal Investigator, Associate Professor, Department of Biology, East Carolina University
Dr. Mario Muscarella (website): Assistant Professor, University of Alaska Fairbanks