Estimate the transform matrix between x and y channels. The example data: data151_xch is thunderstorm estimation results for beads in x channel. data151_ych is thunderstorm estimation results for beads in y channel. Note, we don’t have to flip the y channel during the thunderstorm process
crop and save tiff images based on estimated tform.m This code allows separate a big FoV into multiple small FoVs.
Estimate the background by subtract pixels with SMs. Similarly, thunderstorm estimated results are used to determine the position of SM for subtracting.