This repository contains MATLAB scripts and utilities for the analysis of simulated and experimental POLCAM single-molecule orientation-localisation microscopy (SMOLM) data using RoSE-O, providing accurate and precise measurements of the location and orientation of single molecules.
Paper: [POLCAM: Instant molecular orientation microscopy for the life sciences](paper link here)
bioRxiv preprint: POLCAM: Instant molecular orientation microscopy for the life sciences
utils\: Utility functions for the RoSE-O analysis, including background estimation, basis image computation, and the core RoSE-O analysis algorithm.RoSEO_PolCam_simData_demo.m: Simulate POLCAM data using predefined parameters, analyze the data using RoSE-O, and evaluate the detection performance and estimation accuracy and precision.RoSEO_PolCam_fibrils_demo.m: Analyze experimental POLCAM-SMOLM images of amyloid fibrils using RoSE-O. The raw images required for this analysis are available in the data repository.- Sample data required to run this code is available from the Lew Lab OSF repository:
fibrils_NileRed_0.tif: https://osf.io/download/65ededc74dfd8c0b0b27fcf2/fibrils_NileRed_n45.tif: https://osf.io/download/65eded5a8992ec0b57788b41/fibrils_NileRed_p45.tif: https://osf.io/download/65eded8b4dfd8c0b1227f52b/fibrils_NileRed_90.tif: https://osf.io/download/65eded87e5e51c0b6ebc5ef6/
RRID:SCR_025340