Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males

Figure 2C, D

• DeepLabCut model for tracking the flies position : male_copulation_posture_model
• Calculating the male copulation posture : reshape_dataset.ipynb
• Plotting and statistics analysis : piezo_ratio_and_bunsan.R

Figure 2E

• DeepLabCut model for tracking the flies position : male_copulation_posture_model
• Calculating the male copulation posture and plotting : reshape_dataset.ipynb

Figure 3A

• Plotting and statistics analysis : cop_ratio.R

Figure 3B

• Plotting and statistics analysis : cop_ratio.R

Figure 3C

• Plotting and statistics analysis : cop_ratio.R

Figure 5C, H

• Plotting and statistics analysis : copulation_opt.R

Figure 5F

• DeepLabCut model for tracking the flies position : male_copulation_posture_movement_model
• Calculating the male copulation position : laser_opt_analysis.ipynb
• Plotting and statistics analysis : laser_analysis.R

Figure 5G

• DeepLabCut model for tracking the flies position : male_copulation_posture_movement_model
• Calculating the male copulation position : laser_opt_analysis.ipynb
• Plotting and statistics analysis : laser_analysis.R

Figure S2A-K

• DeepLabCut model for tracking the flies position : male_copulation_posture_model
• Calculating the male copulation posture : reshape_dataset_20-30.ipynb
• Plotting and statistics : ratio_graph_drawing.R

Figure S3A

• Plotting and statistics : kick_copulation_analysis.R

Figure S3B

• Plotting and statistics : genital_touch_count_analysis.R

Figure S4A

• Plotting and statistics : activity_analysis.R

Figure S4B

• Plotting and statistics : fly_scale_analysis.R

Figure S5

• Plotting and statistics : copulation_mixing_assay.R

Figure S7A, B

• Plotting and statistics : copulation_opt_head_grooming.R

Figure S9B

• Calculating the male copulation posture : laser_map.ipynb
• Plotting and statistics :laser_strength_analysis.R

Figure S14B

• DeepLabCut model for tracking the flies position : male_copulation_posture_model
• Calculating the DLC time : reshape_dataset.ipynb
• Plotting and statistics : piezo_ratio_and_bunsan.R

Closed-optogenetic system for optogenetic assays with LED

• DeepLabCut model : copulation_detection_model_for_LED
• Bonsai script : copulation_detect_LED.bonsai
• Arduino script : Create_PWMstimu_bonsai_to_arduino_LED.ino, StandardFirmata.ino

Closed-optogenetic system for optogenetic assays with a laser

• DeepLabCut model : copulation_detection_model_for_laser
• Bonsai script : copulation_detect_laser.bonsai
• Arduino script : Create_PWMstimu_bonsai_to_arduino_laser.ino, StandardFirmata.ino