🧠 PatchMaster Analysis Toolkit (Igor Pro)

Custom Igor Pro toolkit for analyzing electrophysiological recordings exported from PatchMaster.

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🚀 Features

• Batch processing of traces using prefix-based selection
• Baseline correction and linear drift removal
• Peak amplitude extraction (corrected and raw)
• Passive membrane property estimation (Cm, Rs)
• Organized output structure for downstream analysis

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⚙️ Installation

1. Copy all .ipf files into your Igor Pro User Procedures folder

2. Restart Igor Pro (or recompile procedures)

3. Open the panel from the menu:

Analysis → Nanion → Panel

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🧩 Workflow

The core analysis pipeline follows these steps:

Trace selection → Baseline correction → Linear detrending → Peak extraction → Passive property estimation

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▶️ Example Usage

1. Export data from PatchMaster

Experiments must be exported from PatchMaster using the following settings:

• Export one group at a time
• Format: Igor
• Enable:
  • Trace relative to sweep
  • Allow data access
• Export mode: Traces and Stimulus

These settings ensure compatibility with the analysis pipeline and proper reconstruction of traces in Igor Pro.

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2. Load data in Igor Pro

• Open the exported file by double-clicking the PatchMaster-generated file (.pxp)
• This will load all waves into the root of the Data Browser (typically unorganized)

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3. Define trace prefix

• The prefix corresponds to the PatchMaster group name
• All matching traces will be selected for analysis

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4. 📁 Folder Structure

Root/

├─ Trace/ # Raw traces

├─ Stimulus / # Stimulus

├─ Analysis/ # Output results

└─ Packages/

└─ Wave_prefix

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5. Output

Results are saved in the Analysis/ folder:

• peak_net → corrected peak amplitudes.
• Netpeak → [corrected, fit, raw] values.
• cm → membrane capacitance.
• rs → series resistance.
• Netpeak_index → index of maximum response.

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⚠️ Design Notes

• Some dependencies (e.g., Wave_prefix) are intentionally implicit
• Folder structure is partially hardcoded to match experimental organization
• Certain legacy functions are preserved separately for reference

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🛠️ Requirements

• Igor Pro
• PatchMaster-exported data
• Consistent trace naming convention

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🔮 Future Improvements

• Parameterization of folder structure
• Improved validation of dependencies
• Modularization of analysis steps
• Export compatibility (e.g., CSV, HDF5 for Python/R workflows)