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How to deconvolve a tomogram using IMOD

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Ricardo Righetto edited this page Aug 29, 2024 · 2 revisions

A quick way to obtain a denoised tomogram is to deconvolve it using the ad hoc Wiener filter defined in the Warp paper. This filter is now implemented in several software packages, among which IMOD via the mtffilter command since version 4.12.36.

Here is an example of how to deconvolve a tomogram using mtffilter:

mtffilter tomo.rec tomo_deconv.rec -deconv 1.0 -snr 1.0 -defocus 2.0 -pixel 0.784

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