K-Means Clustering Analysis Error: K-Means error and Disconnected from the server

[JL4917]

Hello,
I am trying to do a K-Means Clustering Analysis on my data and when I tried to do the analysis, I received an error: (Error:K-Means error) and I was disconnected from the server.

I have checked the data for alpha diversity, diversity index curve, PCA, PCoA, taxonomical bar chart, and taxonomical heat map. All of them gave great graphs.
Is there a way to see what went wrong?

[raytonghk]

Hi @JL4917,
Is the error reproducible? Can you give more details on what situation the error occur?

[JL4917]

Hello @raytonghk,
Yes it is reproducible.
I am able to click my Project and the Data Label.
I am also able to click any of the selections for Taxonomic Rank For Agglomeration, Abundance Type, and Ordination Method.
However, when I click the Plot button, it disconnects me from the server.
I tried different combinations of Filter By Column, Taxonomic Rank For Agglomeration, Abundance Type, and Ordination Method. When I click the Plot button, it will disconnect me from the server with the (Busy.. hourglass) spinning perpetually.

[raytonghk]

Hi @JL4917,
Thank you for interested in GenePiper and the bug report. The problem was occurred when switching between different ordination methods. And it was fixed. Please update the GenePiper.

[JL4917]

Hello @raytonghk,
After working on Genepiper more, I can provide more information.
I am working with Mothur and I reformatted the output files so that it is similar to the sample files that you provided in the sampleData folder.
Genepiper properly recognizes my files when I upload them to the "Import Tables".

All the graphs from alpha diversity through NMDS looks great! I don't seem to have any problems.

However, when I try to graph using K-Means Clustering Analysis, I will be disconnected from the server as I mentioned before.

When I use the sample files from the sampleData folder, I do not run into this issue.

May I send you my files? It may be easier to identify the source of the issue that way.

[raytonghk]

Hi @JL4917,
Yes. You can download your phyloseq rds file in Filter Phyloseq module and send to my email.