diff --git a/Misc/NEWS.d/next/Library/2025-12-06-13-19-43.gh-issue-142207.x_X9oH.rst b/Misc/NEWS.d/next/Library/2025-12-06-13-19-43.gh-issue-142207.x_X9oH.rst
new file mode 100644
index 00000000000000..69ca8c41ac9b8b
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2025-12-06-13-19-43.gh-issue-142207.x_X9oH.rst
@@ -0,0 +1,2 @@
+Fix: profiling.sampling may cause assertion ``!(has_gil &&
+gil_requested)``
diff --git a/Modules/_remote_debugging/threads.c b/Modules/_remote_debugging/threads.c
index 99147b01a1b9ed..83afe07e22809c 100644
--- a/Modules/_remote_debugging/threads.c
+++ b/Modules/_remote_debugging/threads.c
@@ -335,12 +335,10 @@ unwind_stack_for_thread(
 #endif
     if (has_gil) {
         status_flags |= THREAD_STATUS_HAS_GIL;
+        // gh-142207 for remote debugging.
+        gil_requested = 0;
     }
 
-    // Assert that we never have both HAS_GIL and GIL_REQUESTED set at the same time
-    // This would indicate a race condition in the GIL state tracking
-    assert(!(has_gil && gil_requested));
-
     // Check CPU status
     long pthread_id = GET_MEMBER(long, ts, unwinder->debug_offsets.thread_state.thread_id);