Hi Josh. I tried running the latest PixelBased on Seppe's new data 3 times (I didn't clear the previously created tiles/output in the example here, but I did try it earlier). The first and third time I received the following error message that I refer to in the title:
Round 4 tile 18 already done.
Round 4 tile 19 already done.
Round 4 tile 20 already done.
Warning: xypos empty - using values from previous round
> In iss/extract_and_filter (line 79)
In bridge_process_sample1 (line 67)
Error using sub2ind (line 73)
Out of range subscript.
Error in iss/fstack_modified>gauss3P (line 145)
Index1 = sub2ind([M,N,P], IM(:), IN(:), Ic-STEP);
Error in iss/fstack_modified (line 79)
[u, s, A, fmax] = gauss3P(opts.focus, fm);
Error in iss/extract_and_filter (line 249)
I_mod = o.fstack_modified(I);
Error in bridge_process_sample1 (line 67)
o = o.extract_and_filter;
The second time I received a different error message and I followed its directions:
Round 4 tile 18 already done.
Round 4 tile 19 already done.
Round 4 tile 20 already done.
Warning: xypos empty - using values from previous round
> In iss/extract_and_filter (line 79)
In bridge_process_sample1 (line 67)
Error using iss/extract_and_filter (line 229)
Some tiles in imaging rounds already exist, but o.ExtractScale = auto.
This will result in different scalings used for different tiles.
If tiles up to this point were obtained with a manual value of o.ExtractScale,
set o.ExtractScale to this value and rerun.
If tiles up to this point were obtained with auto value, delete tile 8 in round 1 and rerun, tile location:
cache/NLF-133genes/sample1/tiles/r0001_t8.tif.
Error in bridge_process_sample1 (line 67)
o = o.extract_and_filter;
I should point out that Seppe ran into a computer issue during sequencing at Round 4. He actually ended up doing Round 4 twice and this is using his second go at it. So it's possibly a technical issue not related to your software. But if you're not sure what's going on, do you think I should try an earlier commit? Perhaps the one that I had been using on his pilot? Or maybe I can try the current commit on his pilot and see if its goes through?
Hi Josh. I tried running the latest PixelBased on Seppe's new data 3 times (I didn't clear the previously created tiles/output in the example here, but I did try it earlier). The first and third time I received the following error message that I refer to in the title:
The second time I received a different error message and I followed its directions:
I should point out that Seppe ran into a computer issue during sequencing at Round 4. He actually ended up doing Round 4 twice and this is using his second go at it. So it's possibly a technical issue not related to your software. But if you're not sure what's going on, do you think I should try an earlier commit? Perhaps the one that I had been using on his pilot? Or maybe I can try the current commit on his pilot and see if its goes through?