treesapp assign error

[Cayna26]

Hello!

I am a master student in evolutionary biology trying to run treesapp assign on a fasta file containing 11 a.a sequences for the mitochondrial gene ATPase6 (and have tried with other mitochondrial genes but no success), and the following error occures:

Arguments used:
-i ATPase6_diploids.faa --trim_align --num_procs 8 -o /home/cayna/miniconda3/envs/treesapp_cenv/mydata/ATPase6
TreeSAPP will analyze the 11 sequences found in input.
Parsing HMMER domain tables for high-quality matches... done.
WARNING:
No alignments found! TreeSAPP is exiting now.

I really don't understant why this is happening, although the test provided - treesapp assign -i TreeSAPP/tests/test_data/marker_test_suite.faa -m prot --trim_align -o assign_test -t McrA,DsrAB - worked just fine.

I have tried to create a refpkg using the same fasta file containing 11 a.a sequences for the mitochondrial gene ATPase6, to see if it would fix anything but i wasn't successfull because it kept removing all sequences except one because it considered them all equal.

I really don't know how to proceed...
It's my first time using TreeSAPP so i would immensly appreciate your help!

To Reproduce
Steps to reproduce the behavior:

1. Run the command treesapp assign -i ATPase6_diploids.faa --trim_align --num_procs 8 -o ~/miniconda3/envs/treesapp_cenv/mydata/ATPase6
2. See error:

Arguments used:
-i ATPase6_diploids.faa --trim_align --num_procs 8 -o /home/cayna/miniconda3/envs/treesapp_cenv/mydata/ATPase6
TreeSAPP will analyze the 11 sequences found in input.
Parsing HMMER domain tables for high-quality matches... done.
WARNING:
No alignments found! TreeSAPP is exiting now.

3. See file : TreeSAPP_classify_log.txt

System information (please complete the following information):

• OS: Windows subsystem for Linux - Ubuntu 18.04.5
• Python version 3.7.12
• TreeSAPP Version 0.11.4