diff --git a/src/coreclr/jit/objectalloc.cpp b/src/coreclr/jit/objectalloc.cpp
index 012248266bf701..d8751ac07c686a 100644
--- a/src/coreclr/jit/objectalloc.cpp
+++ b/src/coreclr/jit/objectalloc.cpp
@@ -3517,6 +3517,21 @@ void ObjectAllocator::CheckForGuardedAllocationOrCopy(BasicBlock* block,
             RecordAppearance(lclNum, block, stmt, use);
         }
     }
+    else if (!data->IsIntegralConst(0))
+    {
+        // Store into a tracked enumerator local from an unrecognized source
+        // (e.g. a virtual GetEnumerator call that did not devirtualize, into
+        // a local Roslyn shares between two enumerator scopes). Record it so
+        // CheckCanClone's multiple-defs check bails out of unsafe cloning.
+        // Null/zero stores are skipped: the inliner emits these as GC cleanup
+        // of dead temps. See https://github.com/dotnet/runtime/issues/127075.
+        //
+        unsigned pseudoIndex = BAD_VAR_NUM;
+        if (m_EnumeratorLocalToPseudoIndexMap.TryGetValue(lclNum, &pseudoIndex))
+        {
+            RecordAppearance(lclNum, block, stmt, use);
+        }
+    }
 }
 
 //------------------------------------------------------------------------------
diff --git a/src/tests/JIT/Regression/JitBlue/Runtime_127075/Runtime_127075.cs b/src/tests/JIT/Regression/JitBlue/Runtime_127075/Runtime_127075.cs
new file mode 100644
index 00000000000000..8120052d1875ed
--- /dev/null
+++ b/src/tests/JIT/Regression/JitBlue/Runtime_127075/Runtime_127075.cs
@@ -0,0 +1,51 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+
+using System;
+using System.Collections.Generic;
+using System.Runtime.CompilerServices;
+using System.Threading;
+using Xunit;
+
+public class Runtime_127075
+{
+    public interface I;
+    public sealed class A : I;
+    public sealed class B : I;
+
+    // Roslyn lowers this collection literal stored to IReadOnlyCollection<I>
+    // into a `new <>z__ReadOnlyArray<I>(new I[]{...})` wrapper. The wrapper's
+    // GetEnumerator() does NOT devirtualize even under PGO (the array element
+    // type is inexact), so it stays as a virtual call. That is what triggered
+    // the bug: the second loop's enumerator local store was hidden from
+    // conditional escape analysis.
+    //
+    private static readonly IReadOnlyCollection<I> s_tail = [new B(), new B()];
+
+    [MethodImpl(MethodImplOptions.NoInlining)]
+    private static I[] Combine(IReadOnlyCollection<I> head)
+    {
+        return [.. head, .. s_tail];
+    }
+
+    [Fact]
+    public static void TestEntryPoint()
+    {
+        I[] head = [new A(), new A(), new A(), new A()];
+        for (int i = 0; i < 300; i++)
+        {
+            I[] result = Combine(head);
+            Assert.Equal(head.Length + s_tail.Count, result.Length);
+            for (int j = 0; j < head.Length; j++)
+            {
+                Assert.Same(head[j], result[j]);
+            }
+            int k = head.Length;
+            foreach (I t in s_tail)
+            {
+                Assert.Same(t, result[k++]);
+            }
+            Thread.Sleep(1);
+        }
+    }
+}
diff --git a/src/tests/JIT/Regression/JitBlue/Runtime_127075/Runtime_127075.csproj b/src/tests/JIT/Regression/JitBlue/Runtime_127075/Runtime_127075.csproj
new file mode 100644
index 00000000000000..88d79098489f98
--- /dev/null
+++ b/src/tests/JIT/Regression/JitBlue/Runtime_127075/Runtime_127075.csproj
@@ -0,0 +1,16 @@
+<Project Sdk="Microsoft.NET.Sdk">
+  <PropertyGroup>
+    <Optimize>True</Optimize>
+    <!-- Needed for CLRTestEnvironmentVariable -->
+    <RequiresProcessIsolation>true</RequiresProcessIsolation>
+  </PropertyGroup>
+  <ItemGroup>
+    <Compile Include="$(MSBuildProjectName).cs" />
+    <!-- The issue reproduces only with tiered compilation and PGO enabled,
+         and with QuickJitForLoops enabled so the spread method is instrumented
+         in Tier-0 before being promoted to Tier-1 with PGO data. -->
+    <CLRTestEnvironmentVariable Include="DOTNET_TieredCompilation" Value="1" />
+    <CLRTestEnvironmentVariable Include="DOTNET_TieredPGO" Value="1" />
+    <CLRTestEnvironmentVariable Include="DOTNET_TC_QuickJitForLoops" Value="1" />
+  </ItemGroup>
+</Project>