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Update README.md
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README.md

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@@ -49,26 +49,26 @@ sigma_tensor = 7/scale # sigma of applied gauss filter / windowsize for
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# 7 um for collagen
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edge = 40 # Cut off pixels at the edge since values at the border cannot be trusted
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segmention_thres = 1.0 # for cell segmentation, thres 1 equals normal otsu threshold , change to detect different percentage of bright pixel
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max_dist = None, # optional: specify the maximal distance around cell center for the analysis (in px)
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max_dist = None # optional: specify the maximal distance around cell center for the analysis (in px)
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seg_gaus1, seg_gaus2 = 0.5,100 # 2 gaus filters used as bandpassfilter for local contrast enhancement; For seg_gaus2 = None a single gauss filter is applied
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max_dist = None, # optional: specify the maximal distance around cell center for analysis (in px)
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regional_max_correction = True, # background correction using regional maxima approach
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show_segmentation = False , # display the segmentation output (script won't run further)
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sigma_first_blur = 0.5 , # slight first bluring of whole image before appplying the structure tensor
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angle_sections = 5 , # size of angle sections in degree
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shell_width = None , # pixel width of distance shells (px-value=um-value/scale)
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manual_segmention = False , # segmentation of mask by manual clicking the cell outline
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plotting = True , # creates and saves individual figures additionally to the excel files
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dpi = 200 , # resolution of figures
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SaveNumpy = False , # saves numpy arrays for later analysis - can create large data files
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norm1=1,norm2 = 99 , # contrast spreading for input images between norm1- and norm2-percentile; values below norm1-percentile are set to zero and
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regional_max_correction = True # background correction using regional maxima approach
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show_segmentation = False # display the segmentation output (script won't run further)
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sigma_first_blur = 0.5 # slight first bluring of whole image before appplying the structure tensor
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angle_sections = 5 # size of angle sections in degree
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shell_width = None # pixel width of distance shells (px-value=um-value/scale)
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manual_segmention = False # segmentation of mask by manual clicking the cell outline
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plotting = True # creates and saves individual figures additionally to the excel files
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dpi = 200 # resolution of figures
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SaveNumpy = False # saves numpy arrays for later analysis - can create large data files
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norm1=1,norm2 = 99 # contrast spreading for input images between norm1- and norm2-percentile; values below norm1-percentile are set to zero and
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# values above norm2-percentile are set to 1
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seg_invert=False, # if segmentation is inverted dark objects are detected inseated of bright objects
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seg_iter = 1, # number of repetitions of binary closing, dilation and filling holes steps
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segmention_method="otsu", # use "otsu", "entropy" or "yen" as segmentation method
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segmention_min_area = 1000, # small bjects below this px-area are removed during cell segmentation
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load_segmentation = False, # if True enter the path of the segementation.npy - file in path_seg
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path_seg = None): # to load a mask
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seg_invert=False # if segmentation is inverted dark objects are detected inseated of bright objects
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seg_iter = 1 # number of repetitions of binary closing, dilation and filling holes steps
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segmention_method="otsu" # use "otsu", "entropy" or "yen" as segmentation method
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segmention_min_area = 1000 # small bjects below this px-area are removed during cell segmentation
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load_segmentation = False # if True enter the path of the segementation.npy - file in path_seg
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path_seg = None # to load a mask
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```
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