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Copy file name to clipboardExpand all lines: README.md
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@@ -49,26 +49,26 @@ sigma_tensor = 7/scale # sigma of applied gauss filter / windowsize for
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# 7 um for collagen
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edge =40# Cut off pixels at the edge since values at the border cannot be trusted
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segmention_thres =1.0# for cell segmentation, thres 1 equals normal otsu threshold , change to detect different percentage of bright pixel
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max_dist =None,# optional: specify the maximal distance around cell center for the analysis (in px)
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max_dist =None# optional: specify the maximal distance around cell center for the analysis (in px)
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seg_gaus1, seg_gaus2 =0.5,100# 2 gaus filters used as bandpassfilter for local contrast enhancement; For seg_gaus2 = None a single gauss filter is applied
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max_dist =None, # optional: specify the maximal distance around cell center for analysis (in px)
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regional_max_correction =True,# background correction using regional maxima approach
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show_segmentation =False,# display the segmentation output (script won't run further)
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sigma_first_blur =0.5,# slight first bluring of whole image before appplying the structure tensor
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angle_sections =5,# size of angle sections in degree
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shell_width =None,# pixel width of distance shells (px-value=um-value/scale)
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manual_segmention =False,# segmentation of mask by manual clicking the cell outline
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plotting =True,# creates and saves individual figures additionally to the excel files
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dpi =200,# resolution of figures
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SaveNumpy =False,# saves numpy arrays for later analysis - can create large data files
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norm1=1,norm2 =99,# contrast spreading for input images between norm1- and norm2-percentile; values below norm1-percentile are set to zero and
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regional_max_correction =True# background correction using regional maxima approach
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show_segmentation =False# display the segmentation output (script won't run further)
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sigma_first_blur =0.5# slight first bluring of whole image before appplying the structure tensor
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angle_sections =5# size of angle sections in degree
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shell_width =None# pixel width of distance shells (px-value=um-value/scale)
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manual_segmention =False# segmentation of mask by manual clicking the cell outline
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plotting =True# creates and saves individual figures additionally to the excel files
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dpi =200# resolution of figures
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SaveNumpy =False# saves numpy arrays for later analysis - can create large data files
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norm1=1,norm2 =99# contrast spreading for input images between norm1- and norm2-percentile; values below norm1-percentile are set to zero and
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# values above norm2-percentile are set to 1
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seg_invert=False,# if segmentation is inverted dark objects are detected inseated of bright objects
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seg_iter =1,# number of repetitions of binary closing, dilation and filling holes steps
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segmention_method="otsu",# use "otsu", "entropy" or "yen" as segmentation method
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segmention_min_area =1000,# small bjects below this px-area are removed during cell segmentation
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load_segmentation =False,# if True enter the path of the segementation.npy - file in path_seg
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path_seg =None):# to load a mask
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seg_invert=False# if segmentation is inverted dark objects are detected inseated of bright objects
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seg_iter =1# number of repetitions of binary closing, dilation and filling holes steps
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segmention_method="otsu"# use "otsu", "entropy" or "yen" as segmentation method
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segmention_min_area =1000# small bjects below this px-area are removed during cell segmentation
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load_segmentation =False# if True enter the path of the segementation.npy - file in path_seg
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