22< body style ="padding:20px;font-family:arial; ">
33< h2 > Summary Section</ h2 >
44
5-
65< div style ="width:500px ">
76The projects selected from the < b > Projects</ b > in
8- the left panel are listed in this Summary Section.
7+ the left panel are listed in this Summary Section.
98The possible functions vary with the state, as listed below:
109
1110< table border ='1 ' cellpadding ='5 ' rules =all >
1211< tr > < td > < b > Function</ b > < td > < b > Description</ b >
1312
1413< tr > < td colspan =2 > < i > If there is any project not loaded to the database, you will see:</ i >
15- < tr > < td valign =top > Load All Projects< td valign =top > Load all projects that have not been loaded yet.
14+ < tr > < td valign =top > Load All Projects< td valign =top > Load all projects that have not been loaded yet.
1615
1716< tr > < td colspan =2 > < i > If a project < b > is not</ b > loaded to the database, you will see:</ i >
1817< tr > < td valign =top > Remove from disk< td valign =top > Remove your files from disk. The project will no longer be shown on the left.
19- < tr > < td valign =top > Load project< td valign =top > Loads the sequence and optional annotations to the database.
18+ < tr > < td valign =top > Load project< td valign =top > Loads the sequence and optional annotations to the database.
2019< tr > < td valign =top > Parameters< td valign =top > This brings up a panel of parameters.
2120Make sure the parameters are set right before loading the project.
2221
2322< tr > < td colspan =2 > < i > If a project < b > is</ b > loaded into the database, you will see:</ i >
2423< tr > < td valign =top > Remove from database< td valign =top > The sequences and annotation will be removed, but the files stay on disk.
25- < tr > < td valign =top > Reload project< td valign =top > Executes "Remove project from database" followed by "Load project".
24+ < tr > < td valign =top > Reload project< td valign =top > Executes "Remove project from database" followed by "Load project".
2625< br > If there are
2726alignment files, you will be asked if you want them removed; if your sequences or grp_prefix (see parameters) have
2827changed, say 'yes' as the alignment needs to be run again.
29- < tr > < td valign =top > Reload annotation< td valign =top > Removes and then load the annotations.
30- < br > The "Alignment & Synteny" commands will
31- recognize that the alignments have already be performed and will only perform the synteny computation. If you
28+ < tr > < td valign =top > Reload annotation< td valign =top > Removes and then load the annotations.
29+ < br > The "Alignment & Synteny" commands will
30+ recognize that the alignments have already be performed and will only perform the synteny computation. If you
3231want the alignments to be re-run, you need to "Clear" each respective cell.
33- < tr > < td valign =top > Parameters< td valign =top > After the project is loaded, you can still change the Summary parameters.
32+ < tr > < td valign =top > Parameters< td valign =top > After the project is loaded, you can still change the Summary parameters.
3433</ table >
3534< p >
3635
3736</ div >
3837
3938< h2 > Available Synteny Section</ h2 >
4039< div style ="width:500px ">
40+ Sequence alignments are performed with
41+ < a href ="http://mummer.sourceforge.net/manual "> MUMmer3</ a > ,
42+ but can be changed to use
43+ < a href ="https://mummer4.github.io "> MUMmer4</ a > (see SyMAP
44+ < a href ="https://csoderlund.github.io/SyMAP/MUMmer.html "> MUMmer</ a > ).
45+ BLAT is used for FPC alignments (FPC alignments are probably obsolete now).
46+
47+ < p >
4148This section shows a table with the status of alignments between
4249the selected projects. (Only projects loaded to the database are shown.)
43- < p >
4450Each cell in the table represents a pair of projects (labeled on the
4551rows and columns) and the cell contains a status code showing
4652 whether or not that pair has been aligned (codes are listed below). Note that the table
4753actually shows each pair twice, but only the lower cells are activated.
4854< p >
49- Clicking on a cell selects that pair of projects (the cell then highlights in yellow).
55+ Clicking on a cell selects that pair of projects (the cell then highlights in yellow).
5056Alignment and synteny may then be computed or viewed for that particular pair, using
5157the function buttons (described below). If no cell is selected, you can still
52- use some of the function buttons, which apply to all the cells.
58+ use some of the function buttons, which apply to all the cells.
5359</ div >
5460
5561< p >
@@ -77,7 +83,7 @@ <h2>Available Synteny Section</h2>
7783 A
7884 </ td >
7985 < td align ="left " valign ="top " width ="550 " >
80- The alignment (MUMmer or BLAT ) has been performed but the anchors have not been
86+ The MUMmer alignment (BLAT for FPC-Seq ) has been performed but the anchors have not been
8187 loaded and the synteny computation has not been run. This status occurs if
8288 a pair is completed but then annotations are re-loaded for one of the projects,
8389 or if the MUMmer files have been added by the user (see website "System Guide" for details).
@@ -91,7 +97,7 @@ <h2>Available Synteny Section</h2>
9197 < td align ="left " valign ="top " width ="550 " >
9298 SyMAP puts an empty file with the name 'all.done' in the /align directory to indicate
9399 all alignments successfully completed. If this file is not present, the alignment
94- may not have completed. In this case, any alignments will be completed and the
100+ may not have completed. In this case, any alignments will be completed and the
95101 synteny run.
96102 </ td >
97103</ tr >
@@ -105,9 +111,9 @@ <h2>Available Synteny Section</h2>
105111</ tr >
106112</ table >
107113< p >
108- < b > CPUs:</ b > Enter the maximum number of CPUs to use for an alignment. SyMAP will use
114+ < b > CPUs:</ b > Enter the maximum number of CPUs to use for an alignment. SyMAP will use
109115up to that number (but may use fewer, depending on the number of sequences being aligned). Alternatively,
110- the number of CPUs may be entered as a command line argument (-p N)
116+ the number of CPUs may be entered as a command line argument (-p N)
111117or entered in the symap.config file; this second option will retain the setting between sessions.
112118< p > < b > Concat:</ b > The chromosomes (and/or scaffolds) are concatenated together for one genome
113119and searched against the chromosomes of the 2nd genome. If this is unchecked, then each chromosome
@@ -128,17 +134,17 @@ <h2>Available Synteny Section</h2>
128134</ tr >
129135< tr >
130136 < td align ="center " valign ="top " colspan =2 >
131- < i > Alignment & Synteny</ i >
137+ < i > Alignment & Synteny</ i >
132138 </ td >
133139</ tr >
134140< tr >
135141 < td align ="left " valign ="top ">
136142 All Pairs
137143 </ td >
138144 < td align ="left " valign ="top " width ="550 " >
139- Run (or complete) the synteny computation for all pairs in the Available Syntenies table.
145+ Run (or complete) the synteny computation for all pairs in the Available Syntenies table.
140146 However, this will not run 'self-syntenies'; those need to be done individually by selecting
141- the pair. Also, a draft sequence can only be aligned to one complete sequence, so those
147+ the pair. Also, a draft sequence can only be aligned to one complete sequence, so those
142148 should also be done individually.
143149 </ td >
144150</ tr >
@@ -147,33 +153,33 @@ <h2>Available Synteny Section</h2>
147153 Selected Pair
148154 </ td >
149155 < td align ="left " valign ="top " width ="550 " >
150- Run (or complete) the alignment & synteny computation for the selected project pair.
151- If the pair is already complete, the button label changes to "Re-do", and
156+ Run (or complete) the alignment & synteny computation for the selected project pair.
157+ If the pair is already complete, the button label changes to "Re-do", and
152158 the function is to re-run the synteny computation only. If you wish to re-run
153- the BLAT or MUMmer alignment also, first use the "Clear Pair" function.
159+ the BLAT or MUMmer alignment also, first use the "Clear Pair" function.
154160 </ td >
155161</ tr >
156162< tr >
157163 < td align ="left " valign ="top ">
158164 Clear Pair
159165 </ td >
160166 < td align ="left " valign ="top " width ="550 " >
161- Fully reset the selected pair, removing its synteny results and its MUMmer or BLAT
162- alignment files.
167+ Fully reset the selected pair, removing its synteny results and its MUMmer or BLAT
168+ alignment files.
163169 </ td >
164170</ tr >
165171< tr >
166172 < td align ="left " valign ="top ">
167173 Parameters
168174 </ td >
169175 < td align ="left " valign ="top " width ="550 " >
170- Set alignment & synteny parameters for the selected pair cell (has its own help window).
176+ Set alignment & synteny parameters for the selected pair cell (has its own help window).
171177 </ td >
172178</ tr >
173179
174180< tr >
175181 < td align ="center " valign ="top " colspan =2 >
176- < i > Display for Selected Pair</ i >
182+ < i > Display for Selected Pair</ i >
177183 </ td >
178184</ tr >
179185< tr >
@@ -190,15 +196,15 @@ <h2>Available Synteny Section</h2>
190196 </ td >
191197 < td align ="left " valign ="top " width ="550 " >
192198 A linear display showing synteny blocks of one project aligned
193- next to the chromosomes of the other.
199+ next to the chromosomes of the other.
194200 </ td >
195201</ tr >
196202< tr >
197203 < td align ="left " valign ="top ">
198204 Circle View
199205 </ td >
200206 < td align ="left " valign ="top " width ="550 " >
201- Circular display (Circos-style)
207+ Circular display (Circos-style)
202208 </ td >
203209</ tr >
204210< tr >
@@ -211,7 +217,7 @@ <h2>Available Synteny Section</h2>
211217</ tr >
212218< tr >
213219 < td align ="center " valign ="top " colspan =2 >
214- < i > Display for All Projects</ i >
220+ < i > Display for All Projects</ i >
215221 </ td >
216222</ tr >
217223< tr >
@@ -226,7 +232,7 @@ <h2>Available Synteny Section</h2>
226232</ tr >
227233< tr >
228234 < td align ="center " valign ="top " colspan =2 >
229- < i > For Seq-to-Seq Pairs</ i >
235+ < i > For Seq-to-Seq Pairs</ i >
230236 </ td >
231237</ tr >
232238< tr >
@@ -238,5 +244,9 @@ <h2>Available Synteny Section</h2>
238244 and other valuable synteny-derived information. This does not work for self-synteny or FPC-to-Seq.
239245 </ td >
240246</ tr >
247+ </ table >
248+ < p >
249+
250+ </ div >
241251</ body >
242- </ html >
252+ </ html >
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