filterbycoverage.sh

#!/bin/bash

usage(){
echo "
Written by Brian Bushnell
Last modified May 3, 2016

Description:  Filters an assembly by contig coverage.
The coverage stats file can be generated by BBMap or Pileup.

Usage:  filterbycoverage.sh in=<assembly> cov=<coverage stats> out=<filtered assembly> mincov=5

Parameters:
in=<file>       File containing input assembly.
cov=<file>      File containing coverage stats generated by pileup.
cov0=<file>     Optional file containing coverage stats before normalization.
out=<file>      Destination of clean output assembly.
outd=<file>     (outdirty) Destination of dirty output containing only removed contigs.
minc=5          (mincov) Discard contigs with lower average coverage.
minp=40         (minpercent) Discard contigs with a lower percent covered bases.
minr=0          (minreads) Discard contigs with fewer mapped reads.
minl=1          (minlength) Discard contigs shorter than this (after trimming).
trim=0          (trimends) Trim the first and last X bases of each sequence.
ratio=0         If cov0 is set, contigs will not be removed unless the coverage ratio (of cov to cov0) is at least this (0 disables it).
ow=t            (overwrite) Overwrites files that already exist.
app=f           (append) Append to files that already exist.
zl=4            (ziplevel) Set compression level, 1 (low) to 9 (max).

Java Parameters:
-Xmx            This will set Java's memory usage, overriding autodetection.
                -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs.
                    The max is typically 85% of physical memory.
-eoom           This flag will cause the process to exit if an out-of-memory
                exception occurs.  Requires Java 8u92+.
-da             Disable assertions.

Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems.
For documentation and the latest version, visit: https://bbmap.org
"
}

if [ -z "$1" ] || [ "$1" = "-h" ] || [ "$1" = "--help" ]; then
	usage
	exit
fi

resolveSymlinks(){
	SCRIPT="$(cd "$(dirname "$0")" && pwd)/$(basename "$0")"
	while [ -h "$SCRIPT" ]; do
		DIR="$(dirname "$SCRIPT")"
		SCRIPT="$(readlink "$SCRIPT")"
		[ "${SCRIPT#/}" = "$SCRIPT" ] && SCRIPT="$DIR/$SCRIPT"
	done
	DIR="$(cd "$(dirname "$SCRIPT")" && pwd)"
	if [ -f "$DIR/bbtools.jar" ]; then
		CP="$DIR/bbtools.jar"
	else
		CP="$DIR/current/"
	fi
}

setEnv(){
	. "$DIR/javasetup.sh"
	. "$DIR/memdetect.sh"

	parseJavaArgs "--xmx=800m" "--xms=800m" "--percent=84" "--mode=auto" "$@"
	setEnvironment
}

launch() {
	CMD="java $EA $EOOM $SIMD $XMX $XMS -cp $CP jgi.FilterByCoverage $@"
	echo "$CMD" >&2
	eval $CMD
}

resolveSymlinks
setEnv "$@"
launch "$@"