diff --git a/plugins/webp-uploads/helper.php b/plugins/webp-uploads/helper.php
index c14b58d36f..99092b9df1 100644
--- a/plugins/webp-uploads/helper.php
+++ b/plugins/webp-uploads/helper.php
@@ -514,3 +514,40 @@ function webp_uploads_get_attachment_file_mime_type( int $attachment_id, string
 	$mime_type = $filetype['type'] ?? get_post_mime_type( $attachment_id );
 	return is_string( $mime_type ) ? $mime_type : '';
 }
+
+/**
+ * Checks if Imagick has AVIF transparency support.
+ *
+ * @since n.e.x.t
+ *
+ * @param string|null $version Optional Imagick version string. If not provided, the version will be retrieved from the Imagick class.
+ * @return bool True if Imagick has AVIF transparency support, false otherwise.
+ */
+function webp_uploads_imagick_avif_transparency_supported( ?string $version = null ): bool {
+	$supported       = false;
+	$imagick_version = $version;
+
+	if ( null === $imagick_version && extension_loaded( 'imagick' ) && class_exists( 'Imagick' ) ) {
+		$imagick_version = Imagick::getVersion();
+		$imagick_version = $imagick_version['versionString'];
+	}
+
+	if ( null !== $imagick_version && '' !== $imagick_version && (bool) preg_match( '/\d+(?:\.\d+)+(?:-\d+)?/', $imagick_version, $matches ) ) {
+		$imagick_version = $matches[0];
+	}
+
+	if ( null === $imagick_version || '' === $imagick_version ) {
+		return false;
+	}
+
+	$supported = version_compare( $imagick_version, '6.9.12-68', '>=' );
+
+	/**
+	 * Filters whether Imagick has AVIF transparency support.
+	 *
+	 * @since n.e.x.t
+	 *
+	 * @param bool $supported Whether AVIF transparency is supported.
+	 */
+	return (bool) apply_filters( 'webp_uploads_imagick_avif_transparency_supported', $supported );
+}
diff --git a/plugins/webp-uploads/settings.php b/plugins/webp-uploads/settings.php
index 80d48bdcc0..12de37042e 100644
--- a/plugins/webp-uploads/settings.php
+++ b/plugins/webp-uploads/settings.php
@@ -153,9 +153,10 @@ static function (): void {
  */
 function webp_uploads_generate_avif_webp_setting_callback(): void {
 
-	$selected       = webp_uploads_get_image_output_format();
-	$avif_supported = webp_uploads_mime_type_supported( 'image/avif' );
-	$webp_supported = webp_uploads_mime_type_supported( 'image/webp' );
+	$selected                    = webp_uploads_get_image_output_format();
+	$avif_supported              = webp_uploads_mime_type_supported( 'image/avif' );
+	$avif_transparency_supported = webp_uploads_imagick_avif_transparency_supported();
+	$webp_supported              = webp_uploads_mime_type_supported( 'image/webp' );
 
 	// If neither format is support, the entire field is not shown.
 	if ( ! $avif_supported && ! $webp_supported ) {
@@ -170,7 +171,7 @@ function webp_uploads_generate_avif_webp_setting_callback(): void {
 	}
 
 	// If only one of the two formats is supported, the dropdown defaults to that type and the other type is disabled.
-	if ( ! $avif_supported && 'avif' === $selected ) {
+	if ( ! ( $avif_supported && $avif_transparency_supported ) && 'avif' === $selected ) {
 		$selected = 'webp';
 	} elseif ( ! $webp_supported && 'webp' === $selected ) {
 		$selected = 'avif';
@@ -178,7 +179,7 @@ function webp_uploads_generate_avif_webp_setting_callback(): void {
 	?>
 	<select name="perflab_modern_image_format" id="perflab_modern_image_format" aria-describedby="perflab_modern_image_format_description">
 		<option value="webp"<?php selected( 'webp', $selected ); ?><?php disabled( ! $webp_supported ); ?>><?php esc_html_e( 'WebP', 'webp-uploads' ); ?></option>
-		<option value="avif"<?php selected( 'avif', $selected ); ?><?php disabled( ! $avif_supported ); ?>><?php esc_html_e( 'AVIF', 'webp-uploads' ); ?></option>
+		<option value="avif"<?php selected( 'avif', $selected ); ?><?php disabled( ! ( $avif_supported && $avif_transparency_supported ) ); ?>><?php esc_html_e( 'AVIF', 'webp-uploads' ); ?></option>
 	</select>
 	<label for="perflab_modern_image_format">
 		<?php esc_html_e( 'Generate images in this format', 'webp-uploads' ); ?>
@@ -201,6 +202,13 @@ function webp_uploads_generate_avif_webp_setting_callback(): void {
 			<p><?php esc_html_e( 'WebP support can only be enabled by your hosting provider, so contact them for more information.', 'webp-uploads' ); ?></p>
 		</div>
 	<?php endif; ?>
+	<?php if ( $avif_supported && ! $avif_transparency_supported ) : ?>
+		<br />
+		<div class="notice notice-warning inline">
+			<p><b><?php esc_html_e( 'AVIF transparency support is not available.', 'webp-uploads' ); ?></b></p>
+			<p><?php esc_html_e( 'Current ImageMagick version does not support transparent AVIF images, so contact your hosting provider for more information.', 'webp-uploads' ); ?></p>
+		</div>
+	<?php endif; ?>
 	<?php
 }
 
diff --git a/plugins/webp-uploads/tests/data/class-testcase.php b/plugins/webp-uploads/tests/data/class-testcase.php
index d8176c1cfb..5120f98a07 100644
--- a/plugins/webp-uploads/tests/data/class-testcase.php
+++ b/plugins/webp-uploads/tests/data/class-testcase.php
@@ -153,4 +153,20 @@ public function mock_frontend_body_hooks(): void {
 		remove_all_actions( 'template_redirect' );
 		do_action( 'template_redirect' );
 	}
+
+	/**
+	 * Mocks AVIF transparency support to force a specific scenario.
+	 *
+	 * @param bool $supported Whether to mock AVIF transparency as supported.
+	 */
+	public function mock_avif_transparency_support( bool $supported ): void {
+		remove_all_filters( 'webp_uploads_imagick_avif_transparency_supported' );
+		add_filter(
+			'webp_uploads_imagick_avif_transparency_supported',
+			static function () use ( $supported ) {
+				return $supported;
+			},
+			1
+		);
+	}
 }
diff --git a/plugins/webp-uploads/tests/test-helper.php b/plugins/webp-uploads/tests/test-helper.php
index 0eaa68e74a..6ff43f2c7e 100644
--- a/plugins/webp-uploads/tests/test-helper.php
+++ b/plugins/webp-uploads/tests/test-helper.php
@@ -678,4 +678,46 @@ public function data_provider_to_test_webp_uploads_sanitize_image_format(): arra
 	public function test_webp_uploads_sanitize_image_format( $input, string $expected ): void {
 		$this->assertSame( $expected, webp_uploads_sanitize_image_format( $input ) );
 	}
+
+	/**
+	 * Data provider for ImageMagick version strings.
+	 *
+	 * @return array<string, array{string, bool}> Test data with version strings and expected support.
+	 */
+	public function data_provider_imagick_versions(): array {
+		return array(
+			'ImageMagick 6.8.9 Q16 x86_64'              => array( 'ImageMagick 6.8.9-9 Q16 x86_64 2018-09-28 https://imagemagick.org/index.php', false ),
+			'ImageMagick 6.9.11 Q16 x86_64'             => array( 'ImageMagick 6.9.11-60 Q16 x86_64 2021-01-01 https://imagemagick.org', false ),
+			'ImageMagick 6.9.12 below minimum revision' => array( 'ImageMagick 6.9.12-27 Q16 x86_64 2021-10-24 https://imagemagick.org', false ),
+			'ImageMagick 6.9.12 just below minimum revision' => array( 'ImageMagick 6.9.12-67 Q16 x86_64 2025-06-01 https://imagemagick.org', false ),
+			'ImageMagick 6.9.12 exact minimum revision' => array( 'ImageMagick 6.9.12-68 Q16 x86_64 2025-06-04 https://imagemagick.org', true ),
+			'ImageMagick 6.9.13 above minimum revision' => array( 'ImageMagick 6.9.13-17 Q16 x86_64', true ),
+			'ImageMagick 7.0.24 above minimum major version' => array( 'ImageMagick 7.0.24 Q16 x86_64', true ),
+			'ImageMagick 7.0.25 above minimum major version' => array( 'ImageMagick 7.0.25 Q16 x86_64', true ),
+			'ImageMagick 7.1.0 Q16-HDRI x86_64'         => array( 'ImageMagick 7.1.0-57 Q16-HDRI x86_64 d68553b17:20221230 https://imagemagick.org', true ),
+			'ImageMagick 7.1.1 Q16 aarch64'             => array( 'ImageMagick 7.1.1-15 Q16 aarch64 98eceff6a:20230729 https://imagemagick.org', true ),
+			'ImageMagick 7.1.2 Q16-HDRI x86_64'         => array( 'ImageMagick 7.1.2-7 Q16-HDRI x86_64 23405 https://imagemagick.org', true ),
+			'Empty string should return false'          => array( '', false ),
+			'Invalid string without version should be false' => array( 'Invalid version string', false ),
+			'String with only text should be false'     => array( 'ImageMagick', false ),
+			'Malformed version string should be false'  => array( 'ImageMagick x.y.z', false ),
+		);
+	}
+
+	/**
+	 * Tests webp_uploads_imagick_avif_transparency_supported checks version correctly.
+	 *
+	 * @dataProvider data_provider_imagick_versions
+	 * @covers ::webp_uploads_imagick_avif_transparency_supported
+	 *
+	 * @param string $version          ImageMagick version string.
+	 * @param bool   $expected_support Expected transparency support result.
+	 */
+	public function test_webp_uploads_imagick_avif_transparency_supported_checks_version( string $version, bool $expected_support ): void {
+		remove_all_filters( 'webp_uploads_imagick_avif_transparency_supported' );
+
+		$result = webp_uploads_imagick_avif_transparency_supported( $version );
+
+		$this->assertSame( $expected_support, $result );
+	}
 }
diff --git a/plugins/webp-uploads/tests/test-settings.php b/plugins/webp-uploads/tests/test-settings.php
index a319433235..2a150c58e7 100644
--- a/plugins/webp-uploads/tests/test-settings.php
+++ b/plugins/webp-uploads/tests/test-settings.php
@@ -5,7 +5,9 @@
  * @package webp-uploads
  */
 
-class Test_WebP_Uploads_Settings extends WP_UnitTestCase {
+use WebP_Uploads\Tests\TestCase;
+
+class Test_WebP_Uploads_Settings extends TestCase {
 
 	/**
 	 * @covers ::webp_uploads_add_media_settings_fields
@@ -43,4 +45,30 @@ public function test_webp_uploads_add_settings_action_link(): void {
 			webp_uploads_add_settings_action_link( $default_action_links )
 		);
 	}
+
+	/**
+	 * @covers ::webp_uploads_generate_avif_webp_setting_callback
+	 */
+	public function test_webp_uploads_generate_avif_webp_setting_callback_disables_avif_and_shows_warning(): void {
+		if ( ! webp_uploads_mime_type_supported( 'image/avif' ) ) {
+			$this->markTestSkipped( 'Mime type image/avif is not supported.' );
+		}
+
+		$this->set_image_output_type( 'avif' );
+		$this->mock_avif_transparency_support( false );
+		$output    = get_echo( 'webp_uploads_generate_avif_webp_setting_callback' );
+		$processor = new WP_HTML_Tag_Processor( $output );
+
+		$this->assertTrue( $processor->next_tag( array( 'tag_name' => 'OPTION' ) ) );
+		$this->assertSame( 'webp', $processor->get_attribute( 'value' ) );
+		$this->assertNotNull( $processor->get_attribute( 'selected' ) );
+
+		$this->assertTrue( $processor->next_tag( array( 'tag_name' => 'OPTION' ) ) );
+		$this->assertSame( 'avif', $processor->get_attribute( 'value' ) );
+		$this->assertNotNull( $processor->get_attribute( 'disabled' ) );
+		$this->assertNull( $processor->get_attribute( 'selected' ) );
+
+		$this->assertStringContainsString( 'AVIF transparency support is not available.', $output );
+		$this->assertStringContainsString( 'Current ImageMagick version does not support transparent AVIF images', $output );
+	}
 }