Hi,
I am getting the following error after vsearch starts on the profile step:
....
2025-10-23 01:16:39,273 INFO: Finished reading and testing FastA files
2025-10-23 01:16:39,333 INFO: Writing sequences to files with each 1000 reads.
2025-10-23 01:16:44,076 INFO: Finished writing sequences to 3962 files.
2025-10-23 01:16:44,076 INFO: Starting alignment
vsearch v2.0.3_linux_x86_64, 755.1GB RAM, 192 cores
https://github.com/torognes/vsearch
Fatal error: File type not recognized.
2025-10-23 01:16:44,083 ERROR: Command vsearch --usearch_global /scratch/sdl1f21/ribopools_contam_seq/01.RawData/oldH2O_ribo_3_taxonomic_profiles.0.pe.fasta --db /home/sdl1f21/.conda/envs/mtags/lib/python3.14/site-packages/mTAGs/db/SILVA-138_NR-97_complink_cons.vsearch.udb --id 0.97 --maxaccepts 1000 --maxrejects 1000 --strand both --userout /scratch/sdl1f21/ribopools_contam_seq/01.RawData/oldH2O_ribo_3_taxonomic_profiles.0.pe.fasta.m8 --userfields query+target+qcov+id+qilo+qihi+tilo+tihi+alnlen+ids+mism+gaps+qstrand --query_cov 0.5 --threads 192 --output_no_hits failed with message: None
2025-10-23 01:16:44,083 INFO: mTAGs finished with error code 1
False
Could you advise which file type you think is not recognised? I have downloaded the mtags databases etc. and can see them in the conda path specified before starting these steps. Left it to run on default settings.
Hi,
I am getting the following error after vsearch starts on the profile step:
....
2025-10-23 01:16:39,273 INFO: Finished reading and testing FastA files
2025-10-23 01:16:39,333 INFO: Writing sequences to files with each 1000 reads.
2025-10-23 01:16:44,076 INFO: Finished writing sequences to 3962 files.
2025-10-23 01:16:44,076 INFO: Starting alignment
vsearch v2.0.3_linux_x86_64, 755.1GB RAM, 192 cores
https://github.com/torognes/vsearch
Fatal error: File type not recognized.
2025-10-23 01:16:44,083 ERROR: Command vsearch --usearch_global /scratch/sdl1f21/ribopools_contam_seq/01.RawData/oldH2O_ribo_3_taxonomic_profiles.0.pe.fasta --db /home/sdl1f21/.conda/envs/mtags/lib/python3.14/site-packages/mTAGs/db/SILVA-138_NR-97_complink_cons.vsearch.udb --id 0.97 --maxaccepts 1000 --maxrejects 1000 --strand both --userout /scratch/sdl1f21/ribopools_contam_seq/01.RawData/oldH2O_ribo_3_taxonomic_profiles.0.pe.fasta.m8 --userfields query+target+qcov+id+qilo+qihi+tilo+tihi+alnlen+ids+mism+gaps+qstrand --query_cov 0.5 --threads 192 --output_no_hits failed with message: None
2025-10-23 01:16:44,083 INFO: mTAGs finished with error code 1
False
Could you advise which file type you think is not recognised? I have downloaded the mtags databases etc. and can see them in the conda path specified before starting these steps. Left it to run on default settings.