I am getting different cell counts every time I run the same script where I use recompute() after adding new gates using gs_pop_add() that are boolean combinations of preexisting gates. This has affected downstream analyses where I look for significant differences between subpopulation frequencies. How do I ensure that the gating is reproducible and consistent?
I am getting different cell counts every time I run the same script where I use
recompute()after adding new gates usinggs_pop_add()that are boolean combinations of preexisting gates. This has affected downstream analyses where I look for significant differences between subpopulation frequencies. How do I ensure that the gating is reproducible and consistent?