From e46bab4f16eb34525bcad4cd4a5641630aa636a2 Mon Sep 17 00:00:00 2001 From: Shi Zhang Date: Wed, 18 Mar 2026 11:39:54 -0400 Subject: [PATCH 1/3] add LFS template and scripts focusing on job arrays --- tools/afvs_prepare_folders.py | 15 +++++- tools/afvs_prepare_workunits.py | 2 + tools/afvs_submit_jobs.py | 78 ++++++++++++++++++++++++++++++++ tools/templates/all.ctrl | 22 ++++++++- tools/templates/template1.lsf.sh | 64 ++++++++++++++++++++++++++ 5 files changed, 179 insertions(+), 2 deletions(-) create mode 100644 tools/templates/template1.lsf.sh diff --git a/tools/afvs_prepare_folders.py b/tools/afvs_prepare_folders.py index dce66e1..f249b16 100755 --- a/tools/afvs_prepare_folders.py +++ b/tools/afvs_prepare_folders.py @@ -217,8 +217,21 @@ def check_parameters(config): if(empty_value(config, 'bash_template')): print("* 'bash_template' must be set if batchsystem is 'bash'") error = 1 + elif(config['batchsystem'] == "lsf"): + if(empty_value(config, 'lsf_template')): + print("* 'lsf_template' must be set if batchsystem is 'lsf'") + error = 1 + if(empty_value(config, 'lsf_cpus')): + print("* 'lsf_cpus' must be set if batchsystem is 'lsf'") + error = 1 + if(empty_value(config, 'lsf_queue')): + print("* 'lsf_queue' must be set if batchsystem is 'lsf'") + error = 1 + if(empty_value(config, 'lsf_job_submission_timeout')): + print("* 'lsf_job_submission_timeout' must be set if batchsystem is 'lsf'") + error = 1 else: - print(f"* batchsystem '{config['batchsystem']}' is not supported. Only awsbatch and slurm are supported") + print(f"* batchsystem '{config['batchsystem']}' is not supported. Only awsbatch, slurm, lsf and bash are supported") if(empty_value(config, 'ligand_library_format')): diff --git a/tools/afvs_prepare_workunits.py b/tools/afvs_prepare_workunits.py index 708a611..2ba17f8 100755 --- a/tools/afvs_prepare_workunits.py +++ b/tools/afvs_prepare_workunits.py @@ -329,6 +329,8 @@ def process(ctx): max_array_job_size = int(config['aws_batch_array_job_size']) elif(config['batchsystem'] == "slurm"): max_array_job_size = int(config['slurm_array_job_size']) + elif(config['batchsystem'] == "lsf"): + max_array_job_size = int(config['lsf_array_job_size']) elif(config['batchsystem'] == "bash"): max_array_job_size = int(config['bash_array_job_size']) diff --git a/tools/afvs_submit_jobs.py b/tools/afvs_submit_jobs.py index 42446cf..1b2b5ab 100755 --- a/tools/afvs_submit_jobs.py +++ b/tools/afvs_submit_jobs.py @@ -173,6 +173,82 @@ def submit_slurm(config, client, current_workunit, jobline): # Slow ourselves down a bit time.sleep(0.1) +def submit_lsf(config, client, current_workunit, jobline): + + # Get the template + try: + with open(config['lsf_template']) as f: + lsf_template = jinja2.Template(f.read()) + except IOError as error: + print(f"Cannot open the lsf_template ({config['lsf_template']})") + raise error + + jobline_str = str(jobline) + + # how many jobs are there that we need to submit? + subjobs_count = len(current_workunit['subjobs']) + + # Where are we putting this file? + batch_workunit_base = Path(config['sharedfs_workunit_path']) / jobline_str + batch_workunit_base.mkdir(parents=True, exist_ok=True) + + # LSF doesn't have a good way to submit an array job with a dependency on the previous one, + # so we will submit each subjob separately and then track the job IDs here. + job_ids = {} + + for subjob_id in range(subjobs_count): + + batch_submit_file = batch_workunit_base / f"submit_{subjob_id}.lsf" + + template_values = { + "job_letter": config['job_letter'], + "job_name": config['job_name'], + "threads_to_use": config['threads_to_use'], + "subjob_id": subjob_id, + "lsf_cpus": config['lsf_cpus'], + "lsf_account": config['lsf_account'], + "lsf_queue": config['lsf_queue'], + "workunit_id": jobline_str, + "job_storage_mode": config['job_storage_mode'], + "job_tgz": current_workunit['download_path'], + "batch_workunit_base": batch_workunit_base.resolve().as_posix() + } + render_output = lsf_template.render(template_values) + + try: + with open(batch_submit_file, "w") as f: + f.write(render_output) + except IOError as error: + print(f"Cannot write the workunit lsf file ({batch_submit_file})") + raise error + + cmd = ["bsub"] + + try: + with open(batch_submit_file, "r") as stdin_file: + ret = subprocess.run(cmd, stdin=stdin_file, capture_output=True, + text=True, timeout=int(config['lsf_job_submission_timeout'])) + except subprocess.TimeoutExpired as err: + raise Exception("timeout on submission to bsub") + + if ret.returncode == 0: + match = re.search( + r'Job <(?P\d+)> is submitted', ret.stdout) + if match: + job_ids[str(subjob_id)] = int(match.groupdict()['value']) + else: + raise Exception("bsub returned, but cannot parse output") + else: + raise Exception(f"bsub did not return successfully: {ret.stderr}") + + # Slow ourselves down a bit + time.sleep(0.1) + + current_workunit['status'] = { + 'af_job_status': 'SUBMITTED', + 'job_name': f"afvs-{config['job_letter']}-{jobline_str}", + 'job_ids': job_ids + } def submit_aws_batch(config, client, current_workunit, jobline): @@ -313,6 +389,8 @@ def process(config, start, stop): submit_slurm(config, client, current_workunit, jobline) elif(submit_type == "bash"): run_bash(config, current_workunit, jobline) + elif(submit_type == "lsf"): + submit_lsf(config, client, current_workunit, jobline) else: print(f"Unknown submit type {submit_type}") diff --git a/tools/templates/all.ctrl b/tools/templates/all.ctrl index 6c7d1b6..cd9e9f6 100644 --- a/tools/templates/all.ctrl +++ b/tools/templates/all.ctrl @@ -22,7 +22,7 @@ program_timeout=90 ************************************************ batchsystem=awsbatch -# Possible values: awsbatch, slurm +# Possible values: awsbatch, slurm, lsf ****** AWS Batch Options (if batchsystem=awsbatch) @@ -92,6 +92,26 @@ slurm_array_job_size=100 slurm_job_submission_timeout=10 # Timeout for submission of slurm jobs +****** LSF Options (if batchsystem=lsf) + +lsf_template=./templates/template1.lsf.sh +# Template for the LSF job + +lsf_account= +# LSF project/account to use (-P). If not set, default is used + +lsf_queue=normal +# Queue to submit the job (-q) + +lsf_cpus=18 +# Number of CPUs per job (-n) + +lsf_job_submission_timeout=10 +# Timeout in seconds for each individual bsub call + +lsf_array_job_size=100 +# Maximum number of subjobs to group under a single workunit + ****** Bash Options (if batchsystem=bash) bash_template=./templates/template1.bash diff --git a/tools/templates/template1.lsf.sh b/tools/templates/template1.lsf.sh new file mode 100644 index 0000000..8487020 --- /dev/null +++ b/tools/templates/template1.lsf.sh @@ -0,0 +1,64 @@ +#!/usr/bin/env bash + +# Copyright (C) 2019 Christoph Gorgulla +# Copyright Amazon.com, Inc. or its affiliates. All Rights Reserved. +# +# This file is part of AdaptiveFlow. +# +# AdaptiveFlow is free software: you can redistribute it and/or modify +# it under the terms of the GNU General Public License as published by +# the Free Software Foundation, either version 2 of the License, or +# (at your option) any later version. +# +# AdaptiveFlow is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. +# +# You should have received a copy of the GNU General Public License +# along with AdaptiveFlow. If not, see . + +# --------------------------------------------------------------------------- +# +# Description: LSF job file. +# +# --------------------------------------------------------------------------- + +# Update the BSUB section if needed for your particular LSF +# installation. If a line starts with "##" (two #s) it will be +# ignored + + +#BSUB -J {{job_letter}}-{{workunit_id}}-{{subjob_id}} +#BSUB -n {{lsf_cpus}} +##BSUB -W 12:00 +##BSUB -M 800 +#BSUB -o {{batch_workunit_base}}/{{subjob_id}}.out +#BSUB -e {{batch_workunit_base}}/{{subjob_id}}.err +#BSUB -q {{lsf_queue}} +#BSUB -P {{lsf_account}} + + +# If you are using a virtualenv, make sure the correct one +# is being activated + +source $HOME/afvs_env/bin/activate + + +# Job Information -- generally nothing in this +# section should be changed +################################################################################## + +export AFVS_WORKUNIT={{workunit_id}} +export AFVS_JOB_STORAGE_MODE={{job_storage_mode}} +export AFVS_WORKUNIT_SUBJOB={{subjob_id}} +export AFVS_TMP_PATH=/dev/shm +export AFVS_CONFIG_JOB_TGZ={{job_tgz}} +export AFVS_TOOLS_PATH=${PWD}/bin +export AFVS_VCPUS={{threads_to_use}} + +################################################################################## + +date +%s > {{batch_workunit_base}}/{{subjob_id}}.start +./templates/afvs_run.py +date +%s > {{batch_workunit_base}}/{{subjob_id}}.end \ No newline at end of file From 8bce55b12eb822036f2048136ca7bd0bb0d6f21d Mon Sep 17 00:00:00 2001 From: Shi Zhang Date: Sun, 7 Jun 2026 22:30:51 -0400 Subject: [PATCH 2/3] lfs tests --- input-files/tyk2_qvina/config.txt | 8 + tools/templates/all.ctrl | 34 +- tools/templates/template1.lsf.sh | 5 +- tools/templates/todo.all | 87610 +--------------------------- 4 files changed, 30 insertions(+), 87627 deletions(-) create mode 100644 input-files/tyk2_qvina/config.txt diff --git a/input-files/tyk2_qvina/config.txt b/input-files/tyk2_qvina/config.txt new file mode 100644 index 0000000..0893c78 --- /dev/null +++ b/input-files/tyk2_qvina/config.txt @@ -0,0 +1,8 @@ +receptor=../input-files/tyk2_qvina/tyk2_protein_clean.pdbqt +center_x=-4.0 +center_y=26.6 +center_z=-30.4 +size_x=22 +size_y=22 +size_z=22 +exhaustiveness=8 diff --git a/tools/templates/all.ctrl b/tools/templates/all.ctrl index cd9e9f6..1679652 100644 --- a/tools/templates/all.ctrl +++ b/tools/templates/all.ctrl @@ -1,6 +1,6 @@ ****** Job Resource Configuration -job_name=test +job_name=tyk2_lsf_test # alphabetic characters (i.e. letters from a-z or A-Z) # Used to describe distinct runs (using the same name will # overwrite data if using S3!) @@ -8,7 +8,7 @@ job_name=test threads_per_docking=1 # How many threads should be used for each docking program. -threads_to_use=16 +threads_to_use=8 # This sets how many processes the main execution loop should be using # to process. This is generally 2x the number of vCPUs or hyperthreads # available on the system it is being run on @@ -21,7 +21,7 @@ program_timeout=90 ** Batch system configuration ************************************************ -batchsystem=awsbatch +batchsystem=lsf # Possible values: awsbatch, slurm, lsf ****** AWS Batch Options (if batchsystem=awsbatch) @@ -100,10 +100,10 @@ lsf_template=./templates/template1.lsf.sh lsf_account= # LSF project/account to use (-P). If not set, default is used -lsf_queue=normal +lsf_queue=standard # Queue to submit the job (-q) -lsf_cpus=18 +lsf_cpus=8 # Number of CPUs per job (-n) lsf_job_submission_timeout=10 @@ -125,7 +125,7 @@ bash_array_job_size=100 ** Storage configuration ************************************************ -data_storage_mode=s3 +data_storage_mode=sharedfs # This mode determines where data is retrieved as part of AFVS # * s3: Job data is stored on S3 object store, which is the required # mode if using AWS Batch. Items under the "S3 Object Store" @@ -135,7 +135,7 @@ data_storage_mode=s3 # same shared filesystem that will allow for both input and output # of data. This only allowed if Slurm is set at the scheduler -job_storage_mode=s3 +job_storage_mode=sharedfs # This mode determines where data is stored after a run # * s3: Job data is stored on S3 object store, which is the required # mode if using AWS Batch. Items under the "S3 Object Store" @@ -146,7 +146,7 @@ job_storage_mode=s3 # of data. This is required if using Slurm or bash -data_collection_addressing_mode=hash +data_collection_addressing_mode=metatranche # If input is placed with the hash addressing mode, then use 'hash'. # otherwise use "metatranche" for the classic addressing mode @@ -187,7 +187,7 @@ object_store_data_collection_prefix=Enamine_REAL_Space_2022q12 ****** Shared Filesystem Settings -collection_folder=/home/ec2-user/collections +collection_folder=/scratch_space/YOUR_CLUSTER_USERNAME/collections # Path to where the collection file (ready-to-dock ligands/docking requirement files) # are stored # * This is used when job_storage_mode=sharedfs or @@ -204,7 +204,7 @@ collection_folder=/home/ec2-user/collections ****** Output information -summary_formats=parquet,csv.gz +summary_formats=csv.gz # Format for summary files that are generated with the score data. # Supported values: # * csv.gz (comma delimited files) @@ -250,7 +250,7 @@ collection_list_file=templates/todo.all # Path to the file that contains the collection data on what should be # processed as part of the workflow -dockings_per_subjob=1000 +dockings_per_subjob=100 # Used as how many dockings should be processed per subjob. In general, # a subjob should take about 20 min to an hour for efficiency. # A reasonable number for this is generally 1000. The length of time @@ -265,7 +265,7 @@ ligand_library_format=pdbqt # For the selected docking program, the user is required to set # this to a compatible value. Eg: for plants, set this to mol2 -dynamic_tranche_filtering=1 +dynamic_tranche_filtering=0 # Supported values: # * 0: Disabled # * 1: Enabled @@ -282,7 +282,7 @@ tempdir_default=/dev/shm ****** Virtual Screening Options -docking_scenario_names= +docking_scenario_names=tyk2_qvina # Names for the docking scenarios, separated by colons # Each docking scenario has one value. Multiple docking scenarios/names have # to be separated by colons ":" and without spaces @@ -293,7 +293,7 @@ docking_scenario_names= # In each of thes e folders must be the file config.txt which is used by the docking method to specify its options # If other input files are required by the docking type, usually specified in the config.txt file, they have to be in the same folder. -docking_scenario_methods= +docking_scenario_methods=qvina02 # For each docking scenario name, a docking method has to be specified # Possible values: qvina02, qvina_w, vina, smina_rigid, smina_flexible, gwovina, adfr, AutodockVina_1.2, AutodockZN # gnina, rDock, M-Dock, MCDock, LigandFit, ledock, gold, iGemDock, idock, GalaxyDock3, autodock_gpu @@ -306,7 +306,7 @@ docking_scenario_methods= # smina_rigid has to be used for rigid docking with smina, while smine_flexible for flexible receptor docking # scoring function can also be combined using the same strategy e.g. docking_scenario_methods=vina:nnscore2.0 -docking_scenario_replicas= +docking_scenario_replicas=1 # Series of integers separated by colons ":" # The number of values has to equal the number of docking methods # specified in the variable "docking_scenario_methods" @@ -316,7 +316,7 @@ docking_scenario_replicas= # possible range: 1-99999 per field/docking method # The docking scenario is comprised of all the docking types and their replicas -docking_scenario_batchsizes= +docking_scenario_batchsizes=1 # Purpose: How many ligands should be processed in a single invocation # of the docking method (most methods do not allow more than 1) # Series of integers separated by colons ":" @@ -334,7 +334,7 @@ docking_scenario_basefolder=../input-files # Base directory for where the docking scenarios are held. Nothing other # than the required files for the docking scenario should be placed here -prescreen_mode=1 +prescreen_mode=0 # Run a subset of the ligands included in the library. Only functional # if library supports the prescreen functionality with .listing # file inside the collection diff --git a/tools/templates/template1.lsf.sh b/tools/templates/template1.lsf.sh index 8487020..1f6f06c 100644 --- a/tools/templates/template1.lsf.sh +++ b/tools/templates/template1.lsf.sh @@ -36,7 +36,7 @@ #BSUB -o {{batch_workunit_base}}/{{subjob_id}}.out #BSUB -e {{batch_workunit_base}}/{{subjob_id}}.err #BSUB -q {{lsf_queue}} -#BSUB -P {{lsf_account}} +{% if lsf_account %}#BSUB -P {{lsf_account}}{% endif %} # If you are using a virtualenv, make sure the correct one @@ -44,6 +44,9 @@ source $HOME/afvs_env/bin/activate +# Ensure we run from the AFVS tools directory regardless of LSF working dir default +cd "{{batch_workunit_base}}/../../../tools" + # Job Information -- generally nothing in this # section should be changed diff --git a/tools/templates/todo.all b/tools/templates/todo.all index 6e2adec..b94b9f8 100644 --- a/tools/templates/todo.all +++ b/tools/templates/todo.all 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-SPARSE10_0087590 1000 -SPARSE10_0087591 1000 -SPARSE10_0087592 1000 -SPARSE10_0087593 1000 -SPARSE10_0087594 1000 -SPARSE10_0087595 1000 -SPARSE10_0087596 1000 -SPARSE10_0087597 1000 -SPARSE10_0087598 1000 -SPARSE10_0087599 1000 -SPARSE10_0087600 1000 -SPARSE10_0087601 1000 -SPARSE10_0087602 1000 -SPARSE10_0087603 1000 -SPARSE10_0087604 1000 -SPARSE10_0087605 1000 -SPARSE10_0087606 1000 -SPARSE10_0087607 1000 -SPARSE10_0087608 182 +SFL_0000001 2245 From f6ccec8207b08b1d34834e54c12235945fae7f51 Mon Sep 17 00:00:00 2001 From: Shi Zhang Date: Sun, 7 Jun 2026 23:01:58 -0400 Subject: [PATCH 3/3] .. --- tools/templates/template1.lsf.sh | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/tools/templates/template1.lsf.sh b/tools/templates/template1.lsf.sh index 1f6f06c..0034151 100644 --- a/tools/templates/template1.lsf.sh +++ b/tools/templates/template1.lsf.sh @@ -42,7 +42,8 @@ # If you are using a virtualenv, make sure the correct one # is being activated -source $HOME/afvs_env/bin/activate +source $(conda info --base)/etc/profile.d/conda.sh +conda activate afvs_env # Ensure we run from the AFVS tools directory regardless of LSF working dir default cd "{{batch_workunit_base}}/../../../tools"