| title | Methods |
|---|---|
| layout | single |
| author_profile | true |
| author | mikheyevlab |
| toc | true |
| toc_label | Table of Contents |
| toc_icon | cog |
We host the protocols as Google Docs, so you see the most up to date version, the same one we are using. Please let if you find any errors, or have suggestions on how we can improve the protocols below please email us.
- Easily automatable procedure for DNA extraction.
- Useful for large-insert libraries for de novo genome sequencing.
- This protocol can be used for degraded DNA
- Citation: Tin et al. PLoS ONE 2014, 9(5): e96793
- Related to the EcoRI RAD-tag protocol above, but used for whole genome shotgun sequencing.
- Citation: Tin et al. PLoS ONE 2014, 9(5): e96793
- Modification of original RAD-tag protocol to reduce error and bias due to PCR amplification.
- Citation: Aird et al. BMC Genomics 2013, 14:7907
- High-throughput protocol related to the EcoRI RAD-tag procedure above. Optimized for processing 96 samples at one using a liquid handling robot.
- Useful for processing large numbers of samples at once.
- Most accurate measure of overall dsDNA concentration.