Determination of association/binding constants from titration data using any system of equilibria
- Numerical simulation of equilibrium concentrations from a set of initial concentrations and a system of equilibria, given the equilibrium constants
- Calculation of binding curves from the equilibrium concentrations, given a matrix of epsilon values
- Fitting of calculated binding curves to experimental binding curves, given initial guesses of epsilon and equilibrium constants
A full description is given in the Jupyter notebook, since it can render maths.
A description of the mathematics used in setting up the system of equilibria, and solving for the equilibrium concentrations, check background.pdf.
