| Well_ID | Sample_Name | Trt | Nest |
|---|---|---|---|
| A01 | A1 | ctrl | 1 |
| A02 | A2 | ctrl | 1 |
| A03 | A3 | ctrl | 1 |
| A04 | A4 | ctrl | 1 |
| A05 | A5 | ctrl | 1 |
| A06 | A6 | ctrl | 1 |
| A07 | A7 | ctrl | 1 |
| A08 | A8 | ctrl | 1 |
| A09 | A9 | ctrl | 1 |
| A10 | A10 | ctrl | 1 |
| A11 | A11 | ctrl | 1 |
| A12 | A12 | ctrl | 1 |
| B01 | A13 | ctrl | 1 |
| B02 | A14 | ctrl | 1 |
| B03 | A16 | ctrl | 1 |
| B04 | A17 | ctrl | 1 |
| B05 | A18 | ctrl | 1 |
| B06 | A19 | ctrl | 1 |
| B07 | B2 | exposed | 2 |
| B08 | B3 | exposed | 2 |
| B09 | B4 | exposed | 2 |
| B10 | B5 | exposed | 2 |
| B11 | B6 | exposed | 2 |
| B12 | B7 | exposed | 2 |
| C01 | B8 | exposed | 2 |
| C02 | B10 | exposed | 2 |
| C03 | C1 | ctrl | 3 |
| C04 | C2 | ctrl | 3 |
| C05 | C3 | ctrl | 3 |
| C06 | C4 | ctrl | 3 |
| C07 | C6 | ctrl | 3 |
| C08 | C7 | ctrl | 3 |
| C09 | C8 | ctrl | 3 |
| C10 | C9 | ctrl | 3 |
| C11 | D1 | exposed | 4 |
| C12 | D2 | exposed | 4 |
| D01 | D5 | exposed | 4 |
| D02 | D6 | exposed | 4 |
| D03 | D7 | exposed | 4 |
| D04 | D8 | exposed | 4 |
| D05 | D9 | exposed | 4 |
| D06 | D10 | exposed | 4 |
| D07 | D11 | exposed | 4 |
| D08 | E1 | exposed | 5 |
| D09 | E2 | exposed | 5 |
| D10 | E5 | exposed | 5 |
| D11 | E7 | exposed | 5 |
| D12 | E8 | exposed | 5 |
| E01 | E9 | exposed | 5 |
| E02 | E10 | exposed | 5 |
| E03 | F1 | exposed | 6 |
| E04 | F2 | exposed | 6 |
| E05 | F3 | exposed | 6 |
| E06 | F4 | exposed | 6 |
| E07 | F5 | exposed | 6 |
| E08 | F6 | exposed | 6 |
| E09 | F7 | exposed | 6 |
| E10 | F8 | exposed | 6 |
| E11 | F9 | exposed | 6 |
| E12 | F10 | exposed | 6 |
Associated Publication: Johnston et al., 2014
- The maize leaf is an ideal system to study plant morphogenesis
- It is divided into a proximal (close to stem) sheath and a distal blade each with distinct developmental patterning.
- Specialized epidermal fringe ligule and auricle structures form at the blade-sheath boundary.
For full metadata: see the NCBI GEO page for GSE61333
The SRX vs sample relation is given here broadly captured from the full metadata file:
| Sample_relation | SRR_ID | Maize_cultivar | Genotype | Sample_description | Sample_title |
|---|---|---|---|---|---|
| SRX699505 | SRR1573504 | B73 | wild-type | all cell layers Leaf blade; Repeat:3 | B-3 |
| SRX699506 | SRR1573505 | B73 | wild-type | all cell layers Leaf blade; Repeat:4 | B-4 |
| SRX699507 | SRR1573506 | B73 | wild-type | all cell layers Leaf blade; Repeat:5 | B-5 |
| SRX699508 | SRR1573507 | B73 | wild-type | all cell layers Leaf ligule; Repeat:3 | L-3 |
| SRX699509 | SRR1573508 | B73 | wild-type | all cell layers Leaf ligule; Repeat:4 | L-4 |
| SRX699510 | SRR1573509 | B73 | wild-type | all cell layers Leaf ligule; Repeat:5 | L-5 |
| SRX699511 | SRR1573510 | B73 | wild-type | all cell layers Leaf sheath; Repeat:3 | S-3 |
| SRX699512 | SRR1573511 | B73 | wild-type | all cell layers Leaf sheath; Repeat:4 | S-4 |
| SRX699513 | SRR1573512 | B73 | wild-type | all cell layers Leaf sheath; Repeat:5 | S-5 |
| SRX699514 | SRR1573513 | B73 | wild-type | Wild-type tissue from P6 leaf primordia all cell layers. Repeat 1 | wtL-1 |
| SRX699515 | SRR1573514 | B73 | wild-type | Wild-type tissue from P6 leaf primordia all cell layers. Repeat 2 | wtL-2 |
| SRX699516 | SRR1573515 | B73 | wild-type | Wild-type tissue from P6 leaf primordia all cell layers. Repeat 3 | wtL-3 |
| SRX699517 | SRR1573516 | B73 | lg1-R | lg1 mutant tissue from P6 leaf primordia all cell layers. Repeat 1 | lg1-1 |
| SRX699518 | SRR1573517 | B73 | lg1-R | lg1 mutant tissue from P6 leaf primordia all cell layers. Repeat 2 | lg1-2 |
| SRX699519 | SRR1573518 | B73 | lg1-R | lg1 mutant tissue from P6 leaf primordia all cell layers. Repeat 3 | lg1-3 |
| SRX699520 | SRR1573519 | B73 | wild-type | adaxial L1 tissue was captured. blade; Repeat:1 | B_L1.1 |
| SRX699521 | SRR1573520 | B73 | wild-type | adaxial L1 tissue was captured. blade; Repeat:2 | B_L1.2 |
| SRX699522 | SRR1573521 | B73 | wild-type | adaxial L1 tissue was captured. blade; Repeat:3 | B_L1.3 |
| SRX699523 | SRR1573522 | B73 | wild-type | adaxial L1 tissue was captured. ligule; Repeat:1 | L_L1.1 |
| SRX699524 | SRR1573523 | B73 | wild-type | adaxial L1 tissue was captured. ligule; Repeat:2 | L_L1.2 |
| SRX699525 | SRR1573524 | B73 | wild-type | adaxial L1 tissue was captured. ligule; Repeat:3 | L_L1.3 |
| SRX699526 | SRR1573525 | B73 | wild-type | adaxial L1 tissue was captured. sheath; Repeat:1 | S_L1.1 |
| SRX699527 | SRR1573526 | B73 | wild-type | adaxial L1 tissue was captured. sheath; Repeat:2 | S_L1.2 |
| SRX699528 | SRR1573527 | B73 | wild-type | adaxial L1 tissue was captured. sheath; Repeat:3 | S_L1.3 |
For more details refer to the figure below:
This shows how the various tissue samples were microdissected. This is modified from original Fig 2 in Johnston et al. 2014,
Reads are unstranded.