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Nuclei_Speckle_Counting.cppipe
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359 lines (338 loc) · 31.9 KB
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CellProfiler Pipeline: http://www.cellprofiler.org
Version:4
DateRevision:319
GitHash:
ModuleCount:21
HasImagePlaneDetails:False
Images:[module_num:1|svn_version:\'Unknown\'|variable_revision_number:2|show_window:True|notes:\x5B\'To begin creating your project, use the Images module to compile a list of files and/or folders that you want to analyze. You can also specify a set of rules to include only the desired files in your selected folders.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
:\xff\xfe
Filter images?:\xff\xfeN\x00o\x00 \x00f\x00i\x00l\x00t\x00e\x00r\x00i\x00n\x00g\x00
Select the rule criteria:\xff\xfea\x00n\x00d\x00 \x00(\x00e\x00x\x00t\x00e\x00n\x00s\x00i\x00o\x00n\x00 \x00d\x00o\x00e\x00s\x00 \x00i\x00s\x00i\x00m\x00a\x00g\x00e\x00)\x00 \x00(\x00f\x00i\x00l\x00e\x00 \x00d\x00o\x00e\x00s\x00 \x00c\x00o\x00n\x00t\x00a\x00i\x00n\x00 \x00"\x00.\x00t\x00i\x00f\x00"\x00)\x00
Metadata:[module_num:2|svn_version:\'Unknown\'|variable_revision_number:5|show_window:True|notes:\x5B\'The Metadata module optionally allows you to extract information describing your images (i.e, metadata) which will be stored along with your measurements. This information can be contained in the file name and/or location, or in an external file.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Extract metadata?:\xff\xfeY\x00e\x00s\x00
Metadata data type:\xff\xfeT\x00e\x00x\x00t\x00
Metadata types:\xff\xfe{\x00}\x00
Extraction method count:\xff\xfe1\x00
Metadata extraction method:\xff\xfeE\x00x\x00t\x00r\x00a\x00c\x00t\x00 \x00f\x00r\x00o\x00m\x00 \x00i\x00m\x00a\x00g\x00e\x00 \x00f\x00i\x00l\x00e\x00 \x00h\x00e\x00a\x00d\x00e\x00r\x00s\x00
Metadata source:\xff\xfeF\x00i\x00l\x00e\x00 \x00n\x00a\x00m\x00e\x00
Regular expression to extract from file name:\xff\xfe^\x00(\x00?\x00P\x00<\x00P\x00l\x00a\x00t\x00e\x00>\x00.\x00*\x00)\x00_\x00(\x00?\x00P\x00<\x00W\x00e\x00l\x00l\x00>\x00\x5B\x00A\x00-\x00P\x00\x5D\x00\x5B\x000\x00-\x009\x00\x5D\x00{\x002\x00}\x00)\x00_\x00s\x00(\x00?\x00P\x00<\x00S\x00i\x00t\x00e\x00>\x00\x5B\x000\x00-\x009\x00\x5D\x00)\x00_\x00w\x00(\x00?\x00P\x00<\x00C\x00h\x00a\x00n\x00n\x00e\x00l\x00N\x00u\x00m\x00b\x00e\x00r\x00>\x00\x5B\x000\x00-\x009\x00\x5D\x00)\x00
Regular expression to extract from folder name:\xff\xfe(\x00?\x00P\x00<\x00D\x00a\x00t\x00e\x00>\x00\x5B\x000\x00-\x009\x00\x5D\x00{\x004\x00}\x00_\x00\x5B\x000\x00-\x009\x00\x5D\x00{\x002\x00}\x00_\x00\x5B\x000\x00-\x009\x00\x5D\x00{\x002\x00}\x00)\x00$\x00
Extract metadata from:\xff\xfeA\x00l\x00l\x00 \x00i\x00m\x00a\x00g\x00e\x00s\x00
Select the filtering criteria:\xff\xfea\x00n\x00d\x00 \x00(\x00f\x00i\x00l\x00e\x00 \x00d\x00o\x00e\x00s\x00 \x00c\x00o\x00n\x00t\x00a\x00i\x00n\x00 \x00"\x00"\x00)\x00
Metadata file location:\xff\xfeE\x00l\x00s\x00e\x00w\x00h\x00e\x00r\x00e\x00.\x00.\x00.\x00\x7C\x00
Match file and image metadata:\xff\xfe\x5B\x00\x5D\x00
Use case insensitive matching?:\xff\xfeN\x00o\x00
Metadata file name:\xff\xfe
NamesAndTypes:[module_num:3|svn_version:\'Unknown\'|variable_revision_number:8|show_window:True|notes:\x5B\'The NamesAndTypes module allows you to assign a meaningful name to each image by which other modules will refer to it.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Assign a name to:\xff\xfeI\x00m\x00a\x00g\x00e\x00s\x00 \x00m\x00a\x00t\x00c\x00h\x00i\x00n\x00g\x00 \x00r\x00u\x00l\x00e\x00s\x00
Select the image type:\xff\xfeC\x00o\x00l\x00o\x00r\x00 \x00i\x00m\x00a\x00g\x00e\x00
Name to assign these images:\xff\xfeI\x00m\x00a\x00g\x00e\x00s\x00
Match metadata:\xff\xfe\x5B\x00\x5D\x00
Image set matching method:\xff\xfeO\x00r\x00d\x00e\x00r\x00
Set intensity range from:\xff\xfeI\x00m\x00a\x00g\x00e\x00 \x00m\x00e\x00t\x00a\x00d\x00a\x00t\x00a\x00
Assignments count:\xff\xfe2\x00
Single images count:\xff\xfe0\x00
Maximum intensity:\xff\xfe2\x005\x005\x00.\x000\x00
Process as 3D?:\xff\xfeN\x00o\x00
Relative pixel spacing in X:\xff\xfe1\x00.\x000\x00
Relative pixel spacing in Y:\xff\xfe1\x00.\x000\x00
Relative pixel spacing in Z:\xff\xfe1\x00.\x000\x00
Select the rule criteria:\xff\xfea\x00n\x00d\x00 \x00(\x00m\x00e\x00t\x00a\x00d\x00a\x00t\x00a\x00 \x00d\x00o\x00e\x00s\x00 \x00C\x00 \x00"\x000\x00"\x00)\x00
Name to assign these images:\xff\xfeD\x00A\x00P\x00I\x00
Name to assign these objects:\xff\xfeC\x00e\x00l\x00l\x00
Select the image type:\xff\xfeG\x00r\x00a\x00y\x00s\x00c\x00a\x00l\x00e\x00 \x00i\x00m\x00a\x00g\x00e\x00
Set intensity range from:\xff\xfeI\x00m\x00a\x00g\x00e\x00 \x00m\x00e\x00t\x00a\x00d\x00a\x00t\x00a\x00
Maximum intensity:\xff\xfe2\x005\x005\x00.\x000\x00
Select the rule criteria:\xff\xfea\x00n\x00d\x00 \x00(\x00m\x00e\x00t\x00a\x00d\x00a\x00t\x00a\x00 \x00d\x00o\x00e\x00s\x00 \x00C\x00 \x00"\x002\x00"\x00)\x00
Name to assign these images:\xff\xfeS\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Name to assign these objects:\xff\xfeN\x00u\x00c\x00l\x00e\x00u\x00s\x00
Select the image type:\xff\xfeG\x00r\x00a\x00y\x00s\x00c\x00a\x00l\x00e\x00 \x00i\x00m\x00a\x00g\x00e\x00
Set intensity range from:\xff\xfeI\x00m\x00a\x00g\x00e\x00 \x00m\x00e\x00t\x00a\x00d\x00a\x00t\x00a\x00
Maximum intensity:\xff\xfe2\x005\x005\x00.\x000\x00
Groups:[module_num:4|svn_version:\'Unknown\'|variable_revision_number:2|show_window:True|notes:\x5B\'The Groups module optionally allows you to split your list of images into image subsets (groups) which will be processed independently of each other. Examples of groupings include screening batches, microtiter plates, time-lapse movies, etc.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Do you want to group your images?:\xff\xfeN\x00o\x00
grouping metadata count:\xff\xfe1\x00
Metadata category:\xff\xfeN\x00o\x00n\x00e\x00
IdentifyObjectsManually:[module_num:5|svn_version:\'Unknown\'|variable_revision_number:2|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeD\x00A\x00P\x00I\x00
Name the objects to be identified:\xff\xfeR\x00O\x00I\x00
MaskImage:[module_num:6|svn_version:\'Unknown\'|variable_revision_number:3|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeD\x00A\x00P\x00I\x00
Name the output image:\xff\xfeM\x00a\x00s\x00k\x00D\x00A\x00P\x00I\x00
Use objects or an image as a mask?:\xff\xfeO\x00b\x00j\x00e\x00c\x00t\x00s\x00
Select object for mask:\xff\xfeR\x00O\x00I\x00
Select image for mask:\xff\xfeN\x00o\x00n\x00e\x00
Invert the mask?:\xff\xfeN\x00o\x00
IdentifyPrimaryObjects:[module_num:7|svn_version:\'Unknown\'|variable_revision_number:13|show_window:True|notes:\x5B\'Identify the nuclei from the nuclear stain image. \'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeM\x00a\x00s\x00k\x00D\x00A\x00P\x00I\x00
Name the primary objects to be identified:\xff\xfeN\x00u\x00c\x00l\x00e\x00i\x00
Typical diameter of objects, in pixel units (Min,Max):\xff\xfe5\x00,\x003\x000\x00
Discard objects outside the diameter range?:\xff\xfeY\x00e\x00s\x00
Discard objects touching the border of the image?:\xff\xfeY\x00e\x00s\x00
Method to distinguish clumped objects:\xff\xfeI\x00n\x00t\x00e\x00n\x00s\x00i\x00t\x00y\x00
Method to draw dividing lines between clumped objects:\xff\xfeI\x00n\x00t\x00e\x00n\x00s\x00i\x00t\x00y\x00
Size of smoothing filter:\xff\xfe2\x00
Suppress local maxima that are closer than this minimum allowed distance:\xff\xfe7\x00.\x000\x00
Speed up by using lower-resolution image to find local maxima?:\xff\xfeN\x00o\x00
Fill holes in identified objects?:\xff\xfeA\x00f\x00t\x00e\x00r\x00 \x00b\x00o\x00t\x00h\x00 \x00t\x00h\x00r\x00e\x00s\x00h\x00o\x00l\x00d\x00i\x00n\x00g\x00 \x00a\x00n\x00d\x00 \x00d\x00e\x00c\x00l\x00u\x00m\x00p\x00i\x00n\x00g\x00
Automatically calculate size of smoothing filter for declumping?:\xff\xfeN\x00o\x00
Automatically calculate minimum allowed distance between local maxima?:\xff\xfeN\x00o\x00
Handling of objects if excessive number of objects identified:\xff\xfeC\x00o\x00n\x00t\x00i\x00n\x00u\x00e\x00
Maximum number of objects:\xff\xfe5\x000\x000\x00
Use advanced settings?:\xff\xfeY\x00e\x00s\x00
Threshold setting version:\xff\xfe1\x000\x00
Threshold strategy:\xff\xfeG\x00l\x00o\x00b\x00a\x00l\x00
Thresholding method:\xff\xfeO\x00t\x00s\x00u\x00
Threshold smoothing scale:\xff\xfe0\x00
Threshold correction factor:\xff\xfe1\x00.\x005\x00
Lower and upper bounds on threshold:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Manual threshold:\xff\xfe0\x00.\x000\x00
Select the measurement to threshold with:\xff\xfeN\x00o\x00n\x00e\x00
Two-class or three-class thresholding?:\xff\xfeT\x00w\x00o\x00 \x00c\x00l\x00a\x00s\x00s\x00e\x00s\x00
Assign pixels in the middle intensity class to the foreground or the background?:\xff\xfeB\x00a\x00c\x00k\x00g\x00r\x00o\x00u\x00n\x00d\x00
Size of adaptive window:\xff\xfe5\x000\x00
Lower outlier fraction:\xff\xfe0\x00.\x000\x005\x00
Upper outlier fraction:\xff\xfe0\x00.\x000\x005\x00
Averaging method:\xff\xfeM\x00e\x00a\x00n\x00
Variance method:\xff\xfeS\x00t\x00a\x00n\x00d\x00a\x00r\x00d\x00 \x00d\x00e\x00v\x00i\x00a\x00t\x00i\x00o\x00n\x00
# of deviations:\xff\xfe2\x00.\x000\x00
Thresholding method:\xff\xfeO\x00t\x00s\x00u\x00
IdentifySecondaryObjects:[module_num:8|svn_version:\'Unknown\'|variable_revision_number:10|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input objects:\xff\xfeN\x00u\x00c\x00l\x00e\x00i\x00
Name the objects to be identified:\xff\xfeB\x00i\x00g\x00g\x00e\x00r\x00N\x00u\x00c\x00l\x00e\x00i\x00
Select the method to identify the secondary objects:\xff\xfeD\x00i\x00s\x00t\x00a\x00n\x00c\x00e\x00 \x00-\x00 \x00N\x00
Select the input image:\xff\xfeD\x00A\x00P\x00I\x00
Number of pixels by which to expand the primary objects:\xff\xfe5\x00
Regularization factor:\xff\xfe0\x00.\x000\x005\x00
Discard secondary objects touching the border of the image?:\xff\xfeN\x00o\x00
Discard the associated primary objects?:\xff\xfeN\x00o\x00
Name the new primary objects:\xff\xfeF\x00i\x00l\x00t\x00e\x00r\x00e\x00d\x00N\x00u\x00c\x00l\x00e\x00i\x00
Fill holes in identified objects?:\xff\xfeY\x00e\x00s\x00
Threshold setting version:\xff\xfe1\x000\x00
Threshold strategy:\xff\xfeG\x00l\x00o\x00b\x00a\x00l\x00
Thresholding method:\xff\xfeM\x00i\x00n\x00i\x00m\x00u\x00m\x00 \x00c\x00r\x00o\x00s\x00s\x00 \x00e\x00n\x00t\x00r\x00o\x00p\x00y\x00
Threshold smoothing scale:\xff\xfe0\x00.\x000\x00
Threshold correction factor:\xff\xfe1\x00.\x000\x00
Lower and upper bounds on threshold:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Manual threshold:\xff\xfe0\x00.\x000\x00
Select the measurement to threshold with:\xff\xfeN\x00o\x00n\x00e\x00
Two-class or three-class thresholding?:\xff\xfeT\x00w\x00o\x00 \x00c\x00l\x00a\x00s\x00s\x00e\x00s\x00
Assign pixels in the middle intensity class to the foreground or the background?:\xff\xfeF\x00o\x00r\x00e\x00g\x00r\x00o\x00u\x00n\x00d\x00
Size of adaptive window:\xff\xfe5\x000\x00
Lower outlier fraction:\xff\xfe0\x00.\x000\x005\x00
Upper outlier fraction:\xff\xfe0\x00.\x000\x005\x00
Averaging method:\xff\xfeM\x00e\x00a\x00n\x00
Variance method:\xff\xfeS\x00t\x00a\x00n\x00d\x00a\x00r\x00d\x00 \x00d\x00e\x00v\x00i\x00a\x00t\x00i\x00o\x00n\x00
# of deviations:\xff\xfe2\x00.\x000\x00
Thresholding method:\xff\xfeO\x00t\x00s\x00u\x00
MaskImage:[module_num:9|svn_version:\'Unknown\'|variable_revision_number:3|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeS\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Name the output image:\xff\xfeM\x00a\x00s\x00k\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Use objects or an image as a mask?:\xff\xfeO\x00b\x00j\x00e\x00c\x00t\x00s\x00
Select object for mask:\xff\xfeB\x00i\x00g\x00g\x00e\x00r\x00N\x00u\x00c\x00l\x00e\x00i\x00
Select image for mask:\xff\xfeN\x00o\x00n\x00e\x00
Invert the mask?:\xff\xfeN\x00o\x00
IdentifyPrimaryObjects:[module_num:10|svn_version:\'Unknown\'|variable_revision_number:13|show_window:True|notes:\x5B\'Identify the foci using per-object thresholding to compute a threshold for each individual nuclei object. Some manual adjustment of the smoothing filter size and maxima supression distance is required to optimize segmentation.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeM\x00a\x00s\x00k\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Name the primary objects to be identified:\xff\xfeh\x002\x00a\x00x\x00
Typical diameter of objects, in pixel units (Min,Max):\xff\xfe1\x00,\x005\x00
Discard objects outside the diameter range?:\xff\xfeN\x00o\x00
Discard objects touching the border of the image?:\xff\xfeY\x00e\x00s\x00
Method to distinguish clumped objects:\xff\xfeN\x00o\x00n\x00e\x00
Method to draw dividing lines between clumped objects:\xff\xfeI\x00n\x00t\x00e\x00n\x00s\x00i\x00t\x00y\x00
Size of smoothing filter:\xff\xfe4\x00
Suppress local maxima that are closer than this minimum allowed distance:\xff\xfe4\x00
Speed up by using lower-resolution image to find local maxima?:\xff\xfeY\x00e\x00s\x00
Fill holes in identified objects?:\xff\xfeA\x00f\x00t\x00e\x00r\x00 \x00b\x00o\x00t\x00h\x00 \x00t\x00h\x00r\x00e\x00s\x00h\x00o\x00l\x00d\x00i\x00n\x00g\x00 \x00a\x00n\x00d\x00 \x00d\x00e\x00c\x00l\x00u\x00m\x00p\x00i\x00n\x00g\x00
Automatically calculate size of smoothing filter for declumping?:\xff\xfeY\x00e\x00s\x00
Automatically calculate minimum allowed distance between local maxima?:\xff\xfeY\x00e\x00s\x00
Handling of objects if excessive number of objects identified:\xff\xfeC\x00o\x00n\x00t\x00i\x00n\x00u\x00e\x00
Maximum number of objects:\xff\xfe5\x000\x000\x00
Use advanced settings?:\xff\xfeY\x00e\x00s\x00
Threshold setting version:\xff\xfe1\x000\x00
Threshold strategy:\xff\xfeG\x00l\x00o\x00b\x00a\x00l\x00
Thresholding method:\xff\xfeR\x00o\x00b\x00u\x00s\x00t\x00B\x00a\x00c\x00k\x00g\x00r\x00o\x00u\x00n\x00d\x00
Threshold smoothing scale:\xff\xfe1\x00.\x003\x004\x008\x008\x00
Threshold correction factor:\xff\xfe1\x00.\x000\x00
Lower and upper bounds on threshold:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Manual threshold:\xff\xfe0\x00.\x000\x00
Select the measurement to threshold with:\xff\xfeN\x00o\x00n\x00e\x00
Two-class or three-class thresholding?:\xff\xfeT\x00h\x00r\x00e\x00e\x00 \x00c\x00l\x00a\x00s\x00s\x00e\x00s\x00
Assign pixels in the middle intensity class to the foreground or the background?:\xff\xfeB\x00a\x00c\x00k\x00g\x00r\x00o\x00u\x00n\x00d\x00
Size of adaptive window:\xff\xfe5\x000\x00
Lower outlier fraction:\xff\xfe0\x00.\x006\x00
Upper outlier fraction:\xff\xfe0\x00
Averaging method:\xff\xfeM\x00e\x00a\x00n\x00
Variance method:\xff\xfeS\x00t\x00a\x00n\x00d\x00a\x00r\x00d\x00 \x00d\x00e\x00v\x00i\x00a\x00t\x00i\x00o\x00n\x00
# of deviations:\xff\xfe2\x00
Thresholding method:\xff\xfeO\x00t\x00s\x00u\x00
RelateObjects:[module_num:11|svn_version:\'Unknown\'|variable_revision_number:5|show_window:True|notes:\x5B\'Establish a parent-child between the foci (\\xe2\\x80\\x9cchildren\\xe2\\x80\\x9d) and the nuclei (\\xe2\\x80\\x9cparents\\xe2\\x80\\x9d) in order to determine which foci belong to which nuclei. Then, calculate mean foci measurements for each nucleus.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Parent objects:\xff\xfeB\x00i\x00g\x00g\x00e\x00r\x00N\x00u\x00c\x00l\x00e\x00i\x00
Child objects:\xff\xfeh\x002\x00a\x00x\x00
Calculate child-parent distances?:\xff\xfeN\x00o\x00n\x00e\x00
Calculate per-parent means for all child measurements?:\xff\xfeY\x00e\x00s\x00
Calculate distances to other parents?:\xff\xfeN\x00o\x00
Do you want to save the children with parents as a new object set?:\xff\xfeY\x00e\x00s\x00
Name the output object:\xff\xfeH\x002\x00a\x00X\x00s\x00o\x00r\x00t\x00e\x00d\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
Parent name:\xff\xfeN\x00o\x00n\x00e\x00
RescaleIntensity:[module_num:12|svn_version:\'Unknown\'|variable_revision_number:3|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeD\x00A\x00P\x00I\x00
Name the output image:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00D\x00A\x00P\x00I\x00
Rescaling method:\xff\xfeS\x00t\x00r\x00e\x00t\x00c\x00h\x00 \x00e\x00a\x00c\x00h\x00 \x00i\x00m\x00a\x00g\x00e\x00 \x00t\x00o\x00 \x00u\x00s\x00e\x00 \x00t\x00h\x00e\x00 \x00f\x00u\x00l\x00l\x00 \x00i\x00n\x00t\x00e\x00n\x00s\x00i\x00t\x00y\x00 \x00r\x00a\x00n\x00g\x00e\x00
Method to calculate the minimum intensity:\xff\xfeC\x00u\x00s\x00t\x00o\x00m\x00
Method to calculate the maximum intensity:\xff\xfeC\x00u\x00s\x00t\x00o\x00m\x00
Lower intensity limit for the input image:\xff\xfe0\x00.\x000\x00
Upper intensity limit for the input image:\xff\xfe1\x00.\x000\x00
Intensity range for the input image:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Intensity range for the output image:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Select image to match in maximum intensity:\xff\xfeN\x00o\x00n\x00e\x00
Divisor value:\xff\xfe1\x00.\x000\x00
Divisor measurement:\xff\xfeN\x00o\x00n\x00e\x00
RescaleIntensity:[module_num:13|svn_version:\'Unknown\'|variable_revision_number:3|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the input image:\xff\xfeS\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Name the output image:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Rescaling method:\xff\xfeS\x00t\x00r\x00e\x00t\x00c\x00h\x00 \x00e\x00a\x00c\x00h\x00 \x00i\x00m\x00a\x00g\x00e\x00 \x00t\x00o\x00 \x00u\x00s\x00e\x00 \x00t\x00h\x00e\x00 \x00f\x00u\x00l\x00l\x00 \x00i\x00n\x00t\x00e\x00n\x00s\x00i\x00t\x00y\x00 \x00r\x00a\x00n\x00g\x00e\x00
Method to calculate the minimum intensity:\xff\xfeC\x00u\x00s\x00t\x00o\x00m\x00
Method to calculate the maximum intensity:\xff\xfeC\x00u\x00s\x00t\x00o\x00m\x00
Lower intensity limit for the input image:\xff\xfe0\x00.\x000\x00
Upper intensity limit for the input image:\xff\xfe1\x00.\x000\x00
Intensity range for the input image:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Intensity range for the output image:\xff\xfe0\x00.\x000\x00,\x001\x00.\x000\x00
Select image to match in maximum intensity:\xff\xfeN\x00o\x00n\x00e\x00
Divisor value:\xff\xfe1\x00.\x000\x00
Divisor measurement:\xff\xfeN\x00o\x00n\x00e\x00
GrayToColor:[module_num:14|svn_version:\'Unknown\'|variable_revision_number:3|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select a color scheme:\xff\xfeC\x00o\x00m\x00p\x00o\x00s\x00i\x00t\x00e\x00
Select the image to be colored red:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00D\x00A\x00P\x00I\x00
Select the image to be colored green:\xff\xfeL\x00e\x00a\x00v\x00e\x00 \x00t\x00h\x00i\x00s\x00 \x00b\x00l\x00a\x00c\x00k\x00
Select the image to be colored blue:\xff\xfeM\x00a\x00s\x00k\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Name the output image:\xff\xfeC\x00o\x00l\x00o\x00r\x00I\x00m\x00a\x00g\x00e\x00
Relative weight for the red image:\xff\xfe1\x00.\x000\x00
Relative weight for the green image:\xff\xfe1\x00.\x000\x00
Relative weight for the blue image:\xff\xfe1\x00.\x000\x00
Select the image to be colored cyan:\xff\xfeL\x00e\x00a\x00v\x00e\x00 \x00t\x00h\x00i\x00s\x00 \x00b\x00l\x00a\x00c\x00k\x00
Select the image to be colored magenta:\xff\xfeL\x00e\x00a\x00v\x00e\x00 \x00t\x00h\x00i\x00s\x00 \x00b\x00l\x00a\x00c\x00k\x00
Select the image to be colored yellow:\xff\xfeL\x00e\x00a\x00v\x00e\x00 \x00t\x00h\x00i\x00s\x00 \x00b\x00l\x00a\x00c\x00k\x00
Select the image that determines brightness:\xff\xfeL\x00e\x00a\x00v\x00e\x00 \x00t\x00h\x00i\x00s\x00 \x00b\x00l\x00a\x00c\x00k\x00
Relative weight for the cyan image:\xff\xfe1\x00.\x000\x00
Relative weight for the magenta image:\xff\xfe1\x00.\x000\x00
Relative weight for the yellow image:\xff\xfe1\x00.\x000\x00
Relative weight for the brightness image:\xff\xfe1\x00.\x000\x00
Hidden:\xff\xfe2\x00
Image name:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Color:\xff\xfe#\x00f\x00f\x000\x000\x000\x000\x00
Weight:\xff\xfe1\x00.\x000\x00
Image name:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00D\x00A\x00P\x00I\x00
Color:\xff\xfeb\x00l\x00u\x00e\x00
Weight:\xff\xfe1\x00.\x000\x00
OverlayOutlines:[module_num:15|svn_version:\'Unknown\'|variable_revision_number:4|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Display outlines on a blank image?:\xff\xfeN\x00o\x00
Select image on which to display outlines:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00D\x00A\x00P\x00I\x00
Name the output image:\xff\xfe_\x00N\x00u\x00c\x00l\x00e\x00i\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Outline display mode:\xff\xfeC\x00o\x00l\x00o\x00r\x00
Select method to determine brightness of outlines:\xff\xfeM\x00a\x00x\x00 \x00o\x00f\x00 \x00i\x00m\x00a\x00g\x00e\x00
How to outline:\xff\xfeO\x00u\x00t\x00e\x00r\x00
Select outline color:\xff\xfe#\x00F\x007\x002\x00C\x00F\x00C\x00
Select objects to display:\xff\xfeB\x00i\x00g\x00g\x00e\x00r\x00N\x00u\x00c\x00l\x00e\x00i\x00
OverlayOutlines:[module_num:16|svn_version:\'Unknown\'|variable_revision_number:4|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Display outlines on a blank image?:\xff\xfeN\x00o\x00
Select image on which to display outlines:\xff\xfeR\x00e\x00s\x00c\x00a\x00l\x00e\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00s\x00
Name the output image:\xff\xfe_\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Outline display mode:\xff\xfeC\x00o\x00l\x00o\x00r\x00
Select method to determine brightness of outlines:\xff\xfeM\x00a\x00x\x00 \x00o\x00f\x00 \x00i\x00m\x00a\x00g\x00e\x00
How to outline:\xff\xfeO\x00u\x00t\x00e\x00r\x00
Select outline color:\xff\xfey\x00e\x00l\x00l\x00o\x00w\x00
Select objects to display:\xff\xfeh\x002\x00a\x00x\x00
OverlayOutlines:[module_num:17|svn_version:\'Unknown\'|variable_revision_number:4|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Display outlines on a blank image?:\xff\xfeN\x00o\x00
Select image on which to display outlines:\xff\xfeC\x00o\x00l\x00o\x00r\x00I\x00m\x00a\x00g\x00e\x00
Name the output image:\xff\xfe_\x00B\x00o\x00t\x00h\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Outline display mode:\xff\xfeC\x00o\x00l\x00o\x00r\x00
Select method to determine brightness of outlines:\xff\xfeM\x00a\x00x\x00 \x00o\x00f\x00 \x00i\x00m\x00a\x00g\x00e\x00
How to outline:\xff\xfeO\x00u\x00t\x00e\x00r\x00
Select outline color:\xff\xfey\x00e\x00l\x00l\x00o\x00w\x00
Select objects to display:\xff\xfeh\x002\x00a\x00x\x00
Select outline color:\xff\xfe#\x00D\x00D\x001\x00D\x00E\x002\x00
Select objects to display:\xff\xfeB\x00i\x00g\x00g\x00e\x00r\x00N\x00u\x00c\x00l\x00e\x00i\x00
SaveImages:[module_num:18|svn_version:\'Unknown\'|variable_revision_number:13|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:True]
Select the type of image to save:\xff\xfeI\x00m\x00a\x00g\x00e\x00
Select the image to save:\xff\xfe_\x00B\x00o\x00t\x00h\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Select method for constructing file names:\xff\xfeF\x00r\x00o\x00m\x00 \x00i\x00m\x00a\x00g\x00e\x00 \x00f\x00i\x00l\x00e\x00n\x00a\x00m\x00e\x00
Select image name for file prefix:\xff\xfeD\x00A\x00P\x00I\x00
Enter single file name:\xff\xfeO\x00r\x00i\x00g\x00B\x00l\x00u\x00e\x00
Number of digits:\xff\xfe4\x00
Append a suffix to the image file name?:\xff\xfeY\x00e\x00s\x00
Text to append to the image name:\xff\xfe_\x00B\x00o\x00t\x00h\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Saved file format:\xff\xfet\x00i\x00f\x00f\x00
Output file location:\xff\xfeD\x00e\x00f\x00a\x00u\x00l\x00t\x00 \x00O\x00u\x00t\x00p\x00u\x00t\x00 \x00F\x00o\x00l\x00d\x00e\x00r\x00\x7C\x00Y\x00\x3A\x00\\\x00\\\x00I\x00m\x00a\x00g\x00i\x00n\x00g\x00_\x00D\x00r\x00o\x00p\x00b\x00o\x00x\x00\\\x00\\\x00J\x00i\x00l\x00l\x00y\x00\\\x00\\\x000\x003\x001\x000\x001\x008\x00\\\x00\\\x00R\x00e\x00s\x00u\x00l\x00t\x00s\x00
Image bit depth:\xff\xfe8\x00-\x00b\x00i\x00t\x00 \x00i\x00n\x00t\x00e\x00g\x00e\x00r\x00
Overwrite existing files without warning?:\xff\xfeN\x00o\x00
When to save:\xff\xfeE\x00v\x00e\x00r\x00y\x00 \x00c\x00y\x00c\x00l\x00e\x00
Record the file and path information to the saved image?:\xff\xfeN\x00o\x00
Create subfolders in the output folder?:\xff\xfeN\x00o\x00
Base image folder:\xff\xfeE\x00l\x00s\x00e\x00w\x00h\x00e\x00r\x00e\x00.\x00.\x00.\x00\x7C\x00
SaveImages:[module_num:19|svn_version:\'Unknown\'|variable_revision_number:13|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the type of image to save:\xff\xfeI\x00m\x00a\x00g\x00e\x00
Select the image to save:\xff\xfe_\x00N\x00u\x00c\x00l\x00e\x00i\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Select method for constructing file names:\xff\xfeF\x00r\x00o\x00m\x00 \x00i\x00m\x00a\x00g\x00e\x00 \x00f\x00i\x00l\x00e\x00n\x00a\x00m\x00e\x00
Select image name for file prefix:\xff\xfeD\x00A\x00P\x00I\x00
Enter single file name:\xff\xfeO\x00r\x00i\x00g\x00B\x00l\x00u\x00e\x00
Number of digits:\xff\xfe4\x00
Append a suffix to the image file name?:\xff\xfeY\x00e\x00s\x00
Text to append to the image name:\xff\xfe_\x00N\x00u\x00c\x00l\x00e\x00i\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Saved file format:\xff\xfet\x00i\x00f\x00f\x00
Output file location:\xff\xfeD\x00e\x00f\x00a\x00u\x00l\x00t\x00 \x00O\x00u\x00t\x00p\x00u\x00t\x00 \x00F\x00o\x00l\x00d\x00e\x00r\x00\x7C\x00Y\x00\x3A\x00\\\x00\\\x00I\x00m\x00a\x00g\x00i\x00n\x00g\x00_\x00D\x00r\x00o\x00p\x00b\x00o\x00x\x00\\\x00\\\x00J\x00i\x00l\x00l\x00y\x00\\\x00\\\x000\x003\x001\x000\x001\x008\x00\\\x00\\\x00R\x00e\x00s\x00u\x00l\x00t\x00s\x00
Image bit depth:\xff\xfe8\x00-\x00b\x00i\x00t\x00 \x00i\x00n\x00t\x00e\x00g\x00e\x00r\x00
Overwrite existing files without warning?:\xff\xfeN\x00o\x00
When to save:\xff\xfeE\x00v\x00e\x00r\x00y\x00 \x00c\x00y\x00c\x00l\x00e\x00
Record the file and path information to the saved image?:\xff\xfeN\x00o\x00
Create subfolders in the output folder?:\xff\xfeN\x00o\x00
Base image folder:\xff\xfeE\x00l\x00s\x00e\x00w\x00h\x00e\x00r\x00e\x00.\x00.\x00.\x00\x7C\x00
SaveImages:[module_num:20|svn_version:\'Unknown\'|variable_revision_number:13|show_window:True|notes:\x5B\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the type of image to save:\xff\xfeI\x00m\x00a\x00g\x00e\x00
Select the image to save:\xff\xfe_\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Select method for constructing file names:\xff\xfeF\x00r\x00o\x00m\x00 \x00i\x00m\x00a\x00g\x00e\x00 \x00f\x00i\x00l\x00e\x00n\x00a\x00m\x00e\x00
Select image name for file prefix:\xff\xfeD\x00A\x00P\x00I\x00
Enter single file name:\xff\xfeO\x00r\x00i\x00g\x00B\x00l\x00u\x00e\x00
Number of digits:\xff\xfe4\x00
Append a suffix to the image file name?:\xff\xfeY\x00e\x00s\x00
Text to append to the image name:\xff\xfe_\x00S\x00p\x00e\x00c\x00k\x00l\x00e\x00O\x00u\x00t\x00l\x00i\x00n\x00e\x00s\x00
Saved file format:\xff\xfet\x00i\x00f\x00f\x00
Output file location:\xff\xfeD\x00e\x00f\x00a\x00u\x00l\x00t\x00 \x00O\x00u\x00t\x00p\x00u\x00t\x00 \x00F\x00o\x00l\x00d\x00e\x00r\x00\x7C\x00Y\x00\x3A\x00\\\x00\\\x00I\x00m\x00a\x00g\x00i\x00n\x00g\x00_\x00D\x00r\x00o\x00p\x00b\x00o\x00x\x00\\\x00\\\x00J\x00i\x00l\x00l\x00y\x00\\\x00\\\x000\x003\x001\x000\x001\x008\x00\\\x00\\\x00R\x00e\x00s\x00u\x00l\x00t\x00s\x00
Image bit depth:\xff\xfe8\x00-\x00b\x00i\x00t\x00 \x00i\x00n\x00t\x00e\x00g\x00e\x00r\x00
Overwrite existing files without warning?:\xff\xfeN\x00o\x00
When to save:\xff\xfeE\x00v\x00e\x00r\x00y\x00 \x00c\x00y\x00c\x00l\x00e\x00
Record the file and path information to the saved image?:\xff\xfeN\x00o\x00
Create subfolders in the output folder?:\xff\xfeN\x00o\x00
Base image folder:\xff\xfeE\x00l\x00s\x00e\x00w\x00h\x00e\x00r\x00e\x00.\x00.\x00.\x00\x7C\x00
ExportToSpreadsheet:[module_num:21|svn_version:\'Unknown\'|variable_revision_number:12|show_window:True|notes:\x5B\'Export any measurements to a comma-delimited file (.csv). The measurements made for the nuclei and foci objects will be saved to separate .csv files, in addition to the per-image .csv.\'\x5D|batch_state:array(\x5B\x5D, dtype=uint8)|enabled:True|wants_pause:False]
Select the column delimiter:\xff\xfeC\x00o\x00m\x00m\x00a\x00 \x00(\x00"\x00,\x00"\x00)\x00
Add image metadata columns to your object data file?:\xff\xfeY\x00e\x00s\x00
Select the measurements to export:\xff\xfeY\x00e\x00s\x00
Calculate the per-image mean values for object measurements?:\xff\xfeY\x00e\x00s\x00
Calculate the per-image median values for object measurements?:\xff\xfeN\x00o\x00
Calculate the per-image standard deviation values for object measurements?:\xff\xfeN\x00o\x00
Output file location:\xff\xfeD\x00e\x00f\x00a\x00u\x00l\x00t\x00 \x00O\x00u\x00t\x00p\x00u\x00t\x00 \x00F\x00o\x00l\x00d\x00e\x00r\x00\x7C\x00Y\x00\x3A\x00\\\x00\\\x00I\x00m\x00a\x00g\x00i\x00n\x00g\x00_\x00D\x00r\x00o\x00p\x00b\x00o\x00x\x00\\\x00\\\x00J\x00i\x00l\x00l\x00y\x00\\\x00\\\x000\x003\x001\x000\x001\x008\x00\\\x00\\\x00R\x00e\x00s\x00u\x00l\x00t\x00s\x00
Create a GenePattern GCT file?:\xff\xfeN\x00o\x00
Select source of sample row name:\xff\xfeM\x00e\x00t\x00a\x00d\x00a\x00t\x00a\x00
Select the image to use as the identifier:\xff\xfeN\x00o\x00n\x00e\x00
Select the metadata to use as the identifier:\xff\xfeN\x00o\x00n\x00e\x00
Export all measurement types?:\xff\xfeN\x00o\x00
Press button to select measurements:\xff\xfeI\x00m\x00a\x00g\x00e\x00\x7C\x00C\x00o\x00u\x00n\x00t\x00_\x00h\x002\x00a\x00x\x00,\x00I\x00m\x00a\x00g\x00e\x00\x7C\x00C\x00o\x00u\x00n\x00t\x00_\x00B\x00i\x00g\x00g\x00e\x00r\x00N\x00u\x00c\x00l\x00e\x00i\x00,\x00I\x00m\x00a\x00g\x00e\x00\x7C\x00C\x00o\x00u\x00n\x00t\x00_\x00N\x00u\x00c\x00l\x00e\x00i\x00,\x00I\x00m\x00a\x00g\x00e\x00\x7C\x00F\x00i\x00l\x00e\x00N\x00a\x00m\x00e\x00_\x00D\x00A\x00P\x00I\x00
Representation of Nan/Inf:\xff\xfeN\x00a\x00N\x00
Add a prefix to file names?:\xff\xfeN\x00o\x00
Filename prefix:\xff\xfeM\x00y\x00E\x00x\x00p\x00t\x00_\x00
Overwrite existing files without warning?:\xff\xfeY\x00e\x00s\x00
Data to export:\xff\xfeI\x00m\x00a\x00g\x00e\x00
Combine these object measurements with those of the previous object?:\xff\xfeN\x00o\x00
File name:\xff\xfeD\x00A\x00T\x00A\x00.\x00c\x00s\x00v\x00
Use the object name for the file name?:\xff\xfeY\x00e\x00s\x00