Q_RT_PCR_resources.md

Resources for Quantitative real time PCR (for RNA expression)

Some links to introduce things

We have lots of resources in the lab (dropbox)

Here is a broad overview

ThermoFisher The real time PCR learning center. Also this https://www.thermofisher.com/ca/en/home/life-science/m/pcr/online-real-time-pcr-training.html

ThermoFisher

Also thermoFisher real-time PCR webinars.

Drosophila primers

In addition to looking up published papers that have used primers (those have likely been checked and validated to some degree), for Drosophila melanogaster there is the FlyPrimerBank, the link is here. The paper for it is here.

Notes from Kevin after training on the ThermoFisher StepOne Plus

Slides for in lab training

Assorted Technical information

ThermoFisher RealTime PCR handbook

Analyzing real-time PCR data in the StepOne Plus Software

Avoiding Setup Mistakes in StepOne Plus Software

Normalization in Gene Expression Experiments

How to measure PCR Assay Efficiency?

overview

Primer design

Many tools available (IDT,etc). QuantPrime is well maintained here

things to keep in mind for checking primers

• titration curves and amplification curves to examine efficiency
• melting curves to check for secondary products...

Analysis.