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Description
Hi everyone! I've aligned my fastq files using GRCh38.primary_assembly.genome.fa and now I am launching this command:
/home/valentina/mambaforge/envs/hla-la/opt/hla-la/src/HLA-LA.pl --BAM N77_TCCTGAG_L004.sorted.MD.RG.BQSR.bam --graph PRG_MHC_GRCh38_withIMGT --sampleID N77 --maxThreads 4 --workingDir /data/PROJECTS/GIST/HLA/
HLA-LA.pl
Subsequently, I get the following error:
Identified paths:
samtools_bin: /home/valentina/mambaforge/envs/hla-la/bin/samtools
bwa_bin: /home/valentina/mambaforge/envs/hla-la/bin/bwa
java_bin: /home/valentina/mambaforge/envs/hla-la/bin/java
picard_sam2fastq_bin: /home/valentina/mambaforge/envs/hla-la/bin/picard
General working directory: /data/PROJECTS/LIVIA/GIST_AIRC/HLA
Sample-specific working directory: /data/PROJECTS/LIVIA/GIST_AIRC/HLA/N77
Have found no compatible reference specifications in /home/valentina/mambaforge/envs/hla-la/opt/hla-la/src/../graphs/PRG_MHC_GRCh38_withIMGT/knownReferences - create a file for this BAM file and try again. at /home/valentina/mambaforge/envs/hla-la/opt/hla-la/src/HLA-LA.pl line 364
None of these reference files seems to work:
ls /home/valentina/mambaforge/envs/hla-la/opt/hla-la/src/../graphs/PRG_MHC_GRCh38_withIMGT/knownReferences
1000G_B37_noChr.txt
GEL_B38.txt
1000G_B38.txt
GRCh38.p12.genome.XY_idxstats.txt
Additional_B38_noALTs_withEBV.txt
Illumina_B38.txt
B37_generic_noChr.txt
PRG_MHC_GRCh38_withIMGT.txt
Do you have any advice please? Thanks in advance