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README
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This pipeline will use previously aligned data and will perform those steps:
1- Peak calling (default: macs)
2- Annotation (R's ChIPpeakAnno)
3- Selection of the regions in the proximal promoter
To use the pipeline:
1- Go to the directory where you wish to store the results.
2- Create a file named config.txt. In this file, write the name of the files to analyze.
One (treatment only) or two (treatment and control) file per line.
3- Call the init script situated in this repository:
i.e.: ~/git-clones/ChIP-Seq-Analysis/init
4- Type "make"
If you have many processors avaible, you can use the "-j" option when calling make:
make -j4
Dependencies:
* Rscript (http://stat.ethz.ch/R-manual/R-patched/library/utils/html/Rscript.html)
* MACS (http://liulab.dfci.harvard.edu/MACS/index.html)
* ChIPpeakAnno (http://bioconductor.org/packages/release/bioc/html/ChIPpeakAnno.html)
Note: All the programs must be installed before starting the pipeline. Your PATH environment variable should contain the path to every dependency (except for R packages).